NC State
Wu, B., Zhao, Y., and Gao, P. J. (2006). "A new approach to measurement of saccharifying capacities of crude cellulase," BioRes. 1(2), 189-200


A practical, quantitative approach has been designed, which makes it possible to accurately estimate the saccharifying activities of crude cellulase preparations for insoluble cellulosics. The challenge in activity determination imposed by changes in hydrolysis time and concentration of cellulase and cellulosics on the assay could be overcome by selection of the specific conversion percentage of cellulose as a function of cellulase concentration, that is, the hydrolysis percentage of filter paper by unit cellulase per minute, as the objective function with respect to different concentrations of crude cellulase. A rational and governing equation for crude cellulase assay was derived , and reliable results for quantitatively estimating the saccharifying activities of crude cellulases during the progress of hydrolysis of several cellulosic substrates were obtained.

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