Twelve Pleurotus ostreatus (Jacq. ex Fr.) P. Kumm and six Pleurotus pulmonarius (Fr.) Quél. strains were characterized from the National Culture Collection of Mushrooms, Institute of Botany Kholodny, National Academy of Science, Kyiv, Ukraine (IBK). The strains were grown under commercial conditions on a mixture of wheat straw and sunflower shells under both winter and summer temperatures typical for those climatic conditions. The strains were divided into three groups according to their growing patterns. Important characteristics were compared with a commercial analogue, HK-35, such as vegetative growth, generative growth, and biological efficiency (1.9- to 3.1-fold), and were recorded for strains 2251, 2292, 2316, 2319, and 2320 of P. ostreatus and 2314 of P. pulmonarius. Strains 2251, 2292, 2301, 2321 and 2323 were the most suitable for commercial production, while strains 2319 and 2320 could satisfy processing industry requirements with their high biological efficiency. Strains 2287 and 2317 produced high-quality fruit bodies but probably required a higher temperature for cultivation. Strain 2318 might be attractive for some consumers due to its unique and unusual fruit body shape. Strain 2314 was the most promising for summer cultivation, while strain 537 produced the highest quality fruit bodies.