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Loaiza Rodriguez, J. M., Zamudio, M. A., Lozano, S., Alfaro, A., García, M. T, García, J. C., and López, F. (2021). "Characterization and usage of tule (Typha domingensis Pers) to obtain cellulose pulp in a biorefinery framework," BioResources 16(2), 3328-3347.

Abstract

It was proposed that pre-hydrolysing tule (Typha domingensis Pers) would make it possible to produce handsheets with strength-related properties similar to those of conventional commercial paper, in addition to valorizable hemicelluloses. The hypothesis was tested with a biorefining scheme involving autohydrolysis. Conditions were optimized to maximize the extraction of hemicelluloses while preserving glucan integrity. The autohydrolysis at a liquid to solid ratio of 13 at 141 °C for 45 min (optimized conditions) provided a commercially useful liquor containing primarily tule hemicelluloses. The autohydrolysis of tule before alkaline extraction made it possible to obtain a valuable liquid phase comprising between 23.3% and 71.6% of all hemicelluloses in the raw material. The gross heating value for the hydrolyzed tule was increased (1.6% to 7.9 %) with respect the raw material. The solid phase was subjected to soda–anthraquinone pulping. The yield, ethanol extractives, kappa number, viscosity, glucan, xylan, Klason lignin, soluble lignins, as well as the tensile index were evaluated. Tule was found to be amenable to industrial processing for the production of quality cellulose derivatives. Autohydrolysis provided valorizable liquor in addition to solid that yielded soda–anthraquinone pulp having properties similar to those obtained in its absence, but using milder operating conditions.


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Characterization and Usage of Tule (Typha domingensis Pers) to Obtain Cellulose Pulp in a Biorefinery Framework

Javier M. Loaiza,a,* Minerva A. Zamudio,b Susana, Lozano,a Ascension Alfaro,c Maria T. Garcia,a Juan C. García,a and Francisco Lopez a

It was proposed that pre-hydrolysing tule (Typha domingensis Pers) would make it possible to produce handsheets with strength-related properties similar to those of conventional commercial paper, in addition to valorizable hemicelluloses. The hypothesis was tested with a biorefining scheme involving autohydrolysis. Conditions were optimized to maximize the extraction of hemicelluloses while preserving glucan integrity. The autohydrolysis at a liquid to solid ratio of 13 at 141 °C for 45 min (optimized conditions) provided a commercially useful liquor containing primarily tule hemicelluloses. The autohydrolysis of tule before alkaline extraction made it possible to obtain a valuable liquid phase comprising between 23.3% and 71.6% of all hemicelluloses in the raw material. The gross heating value for the hydrolyzed tule was increased (1.6% to 7.9 %) with respect the raw material. The solid phase was subjected to soda–anthraquinone pulping. The yield, ethanol extractives, kappa number, viscosity, glucan, xylan, Klason lignin, soluble lignins, as well as the tensile index were evaluated. Tule was found to be amenable to industrial processing for the production of quality cellulose derivatives. Autohydrolysis provided valorizable liquor in addition to solid that yielded soda–anthraquinone pulp having properties similar to those obtained in its absence, but using milder operating conditions.

Keywords: Autohydrolysis; Biorefinery; Hemicelluloses; Pulp; Soda–AQ; Tule

Contact information: a: Research Center in Technology of Products and Chemical Processes (PRO²TEC), Department Chemical Engineering, University of Huelva, Huelva 21071 Spain; b: Technological Institute of Ciudad Madero, Ciudad Madero 89460 Mexico; c: Forestry Science Department, University of Huelva, Huelva 21071 Spain; * Corresponding author: javiermauricio.loiza@diq.uhu.es

GRAPHICAL ABSTRACT

INTRODUCTION

The sustainable development of renewable industrial products of high added value is becoming increasingly essential to reverse the growing de-industrialization and delocalization occurring in many countries. To this end, the forest sector and, more specifically, the pulp and paper industry, are searching for alternative, non-wood plant fibres (Mongkhonsiri et al. 2018; Daya and Nourelfath 2019; Pio et al. 2020). In addition, these industries are fostering research into the integral fractionation and biorefining of lignocellulosic materials to obtain a number of products in addition to pulp and energy by using processing schemes similar to those found in oil refining processes (Cherubini 2010; Zhu and Zhuang 2012; Nanda et al. 2015; Cebreiros et al. 2017). In theory, existing schemes for cellulose pulp production could be used at industrial plants for the conversion of lignocellulosic biomass into biorefinable fractions in order to increase profits in the forest and paper industries (Clark et al. 2009; Vila et al. 2011; Pätäri et al. 2016; Rogers et al. 2018).

The world output of paper varies to an extent dependent on the particular type of product. Overall, the production of graphic art paper is on the decline as a result of the increasing usage of digital media in developed countries; in contrast, the production of packaging paper is increasing as a result of the ever-stringent restrictions on plastics (Bajpai 2016). Thus, according to FAO statistics (2018), 57% of all paper and board produced in 2018 (235 million tonnes) was used to manufacture packaging materials. In any case, cellulose pulp production continues to steadily rise and has increased from 400 million tons to 415 million tons, i.e., by 3.85%, in 2018 (FAO 2018). Since 97% of all paper is currently obtained from wood raw materials, there is good reason to explore new sources of lignocellulosic biomass including agricultural and forest residues (Chen et al. 2014; Moriana et al. 2015; Wikberg et al. 2017; Cypriano et al. 2018; FAO 2018; Bian et al. 2019).

The growing interest in increasing the sustainability and decreasing the environmental impact of cellulose pulp and paper production has fostered a search for alternative, non-wood sources of fibre (Khiari et al. 2010; López et al. 2012; Krishnan et al. 2015; Ang et al. 2020). However, only 3% of the inputs for paper production comes from such sources currently (FAO 2018). The alternatives have the advantage of much shorter crop cycles, which is the case with the plant species used as raw material in this study, i.e., tule.

Tule (Typha domingensis Pers) is an aquatic plant native to America that occasionally acts as an invader and forms highly dense colonies known as totorales. Tule is a highly competitive species with a high reproductive rate. A hectare of tule plants can provide an approximate 55 tonnes of green matter up to three times a year (Medina 1959; Morales-Cepeda et al. 2015). This is a rather high biomass production rate relative to conventional forest crops. In addition, the fact that tule roots tend to accumulate heavy metals, nitrates, and phosphates allows metals in water masses to be eliminated by removing the plants (Vera et al. 2010).

The conventional scheme for cellulose pulp production based on alkaline delignification can be upgraded by inserting a previous autohydrolysis step to selectively extract hemicelluloses into the resulting liquor. The liquor, which is rich in hemicellulose polymers (primarily xylo-oligosaccharides and xylose), provides a promising source of products with high added value for usage in the pharmaceutical, chemical, and food industries. In addition, the fermentation medium obtained can be used for fuels and chemicals, e.g., ethanol, furfural, and xylitol (Rivas et al. 2002; García et al. 2011; García-Domínguez et al. 2013; Moniz et al. 2014). Destroying lignocellulosic material by hydrolysis or autohydrolysis has an additional environmental advantage: it facilitates subsequent delignification and avoids the need to use sulphur compounds for this purpose. Treating the hydrolysed raw material with an alkaline reagent, e.g., a soda–anthraquinone or ethanol–soda–anthraquinone mixture, allows cellulose pulp with a low residual lignin content, a high yield and brightness, and good strength-related properties to be obtained (Caparrós et al. 2008; Alfaro et al. 2009, 2010; García et al. 2017; Loaiza et al. 2019).

The effects of a hydrothermal pretreatment on the outcome of the subsequent pulping process has been examined in a wide variety of raw materials. Thus, autohydrolysis has been used prior for the kraft, soda, and ethanol–water pulping of eucalyptus (Loaiza et al. 2016), Leucaena leucocephala K360 (Feria et al. 2011), Arundo donax (Caparros et al. 2007), tagasaste (Chamaecytisus proliferus) (Alfaro et al. 2010), Paulownia fortunei L (Caparrós et al. 2008), and Leucaena diversifolia (Feria et al. 2012). The fact that the properties of the resulting pulp were invariably similar to those obtained without a hydrolysis treatment led the authors to explore a new raw material, i.e., tule (Typha domingensis Pers). In this work, tule was subjected to integral fractionation via autohydrolysis and back-delignification, the autohydrolysis liquor was assessed as a source of monosaccharides and hemicellulose derivatives, and the remaining solid phase was assessed as a raw material for energy, pulp, and paper production.

EXPERIMENTAL

Materials

Characterization and storage of the raw material

Tule plants were obtained from an industrial wastewater receiving body located in the El Conejo lagoon (Altmirade, Tamaulipas region, Mexico). In the present case, it was decided that the plant stems were cut above the water mirror surface. The roots were not of interest, as they usually contain large amounts of metals and nutrients. The raw material was prepared for treatment according to TAPPI standard T257 cm-85 (1999). The stems were chopped to pieces that were approximately 2.5 cm long and stored in air-tight pouches to protect them from ambient moisture (César et al. 2015).

The tule was characterized chemically according to TAPPI standard T264 cm-07 (2007) for its moisture content, TAPPI standard 211 om-02 (2002) for its ash content, and TAPPI standard T204 cm-07 (2007) with Soxhlet extraction (95% ethanol for 5 h) for its ethanol extractives. After characterization, the raw material was ground to a particle size of less than 0.5 mm and subjected to quantitative acid hydrolysis with 72% H2SO4. The resulting hydrolysates were chemically analysed according to TAPPI standard T249 cm-09 (2009). The monomeric sugar content (arabinose, xylose, and glucose) as well as the acetic acid content in the hydrolysis liquor were determined via HPLC (high performance liquid chromatography), using an Aminex HPX-87H ion exchange column at a temperature of 30 °C as the stationary phase and 0.05 M of H2SO4 at a flow rate of 6 mL/min–1 as the mobile phase. The monosaccharide contents were expressed as xylan, araban, or glucan. The Klason and soluble lignin contents were determined according to TAPPI standard T222 om-11 (2011).

Methods

Autohydrolysis

The tule samples and the reaction liquid (water) were mixed in the established proportions and reacted in a 10 L stainless-steel reactor from MK-Systems, Inc. (North Adams, MA) with recycling. The operational conditions for autohydrolysis were as follows: a temperature of 141 °C to 165 °C for 15 min to 45 min, a warm-up time of 43 min, and a liquid to solid ratio of 13 kg of water to 1 kg of raw material. Once the reactor was closed, it was heated and actuated to ensure thorough mixing and uniform swelling of the tule chips. After autohydrolysis, the reactor was cooled down to 25 °C.

Determination of the gross heating values

The gross heating values (constant volume) were determined according to CEN/TS standard 14918 (2005) and UNE standard 16001 EX (2005), using a Parr 6300 automatic isoperibol (bomb) calorimeter and 1.0 g of pellets in each run. For measurements, a cotton thread was attached to a platinum ignition wire and placed in contact with the pellets. The calorimeter bomb (a stainless steel box) was filled with oxygen at a temperature of 25 °C and with 1.0 cm³ of water, and then placed in the isothermal jacket of the calorimeter with an air gap of 10 mm between the surfaces of the jacket and bomb walls. The amount of electrical energy needed for ignition was determined from the potential difference across a 1256 or 2900 μF capacitor discharged from approximately 40 V through a platinum wire. The calorimeter jacket was kept at a constant temperature by circulating water at a temperature of 27 °C.

Pulping

The solid phase from the autohydrolysis process was pulped in the same reactor as the hydrothermal treatment. The reactor was heated and actuated to ensure thorough mixing and uniform swelling of the tule chips. The pretreated raw material was pulped at three different alkali concentrations (14 dry wt%, 17 dry wt%, or 20 dry wt% of NaOH), temperatures (153 °C, 163 °C, or 171 °C) and cooking times (60 min, 90 min, or 120 min), in addition to a fixed liquid to solid ratio (13 to 1) and anthraquinone concentration (0.1 dry wt%). After the pulping time had elapsed, the reactor was cooled down to 25 °C. Following the cooking procedure, the liquor of each pulp sample was filtered off and disintegrated at 2000 rpm for 10 min. The pulping yield, kappa index, and intrinsic viscosity were determined according to TAPPI standard T230 om-99 (1999), TAPPI standard T236 cm-85 (1996), and TAPPI standard T257 cm-85 (1999), respectively. The paper sheets were prepared according to TAPPI standard T205 sp-02 (2014) using a ENJO-F-39.71 sheet machine and assessed for grammage using TAPPI standard T220 sp-01 (2001) and for tensile index using TAPPI standard T494 om-01 (2001).

Experimental design for the autohydrolysis and pulping processes using multiple regression models

The autohydrolysis and pulping processes were modelled and optimized using polynomials comprised of linear and quadratic terms describing the process variables, and their mutual interactions; therefore, the equations established were fit using multiple regressions. A 2n central composite factor design was used to reduce the number of tests needed while ensuring the absence of significant covariances between the dependent variables. This allowed the dependent variables, i.e., the Klason lignin, yield, soluble lignin, glucan, araban, xylan, acetyl groups, and higher heating value, to be related to the independent variables, i.e., the temperature, NaOH concentration, and treatment time.

Modelling required previously normalizing the ranges spanned by the independent variables by applying an equation and identifying the statistically significant influences on the coefficients. This equation was defined as Eq. 1,

(1)

where X is the absolute value of the independent variable concerned, is the average value, and Xmax and Xmin are the maximum and minimum values, respectively. Thus, no term with a p-value greater than 0.05 as per a Student’s t-test or spanning a confidence interval less than 95% wide was included.

The number of tests was calculated according to Eq. 2,

N = 2n + 2·n + nC (2)

where 2n is the number of points constituting the factor design, 2·n is the number of axial points, and nC is the number of central points.

N was 10 for the autohydrolysis process and 16 for the pulping process. The experimental results were fit to the following second-order polynomial equation shown in Eq. 3,

(3)

where X denotes the independent variables and Y denotes the dependent variables and the coefficients ao, bi, ci, and dij are constant characteristics in each model estimated with the experimental data.

The results were assessed with the Statistica software (Version 10.0, StatSoft, Inc., Tulsa, OK). Universal fitting statistics, i.e., R2 and Snedecor’s F-value, were also used. An R2 greater than 0.85 or an F value greater than 5 were taken as acceptable.

To establish a basis for comparison, samples of the untreated tule (without autohydrolysis) were also subjected to soda–anthraquinone delignification under the same pulping conditions as the test that providing the best pulp properties via autohydrolysis, i.e., an alkali concentration of 20%, a temperature of 171 °C, and a cooking time of 120 min.

RESULTS AND DISCUSSION

Chemical Characterization of Tule

The potential of tule as a renewable source for the sustainable production of paper and chemicals (specifically, hemicellulose derivatives) was assessed by comparison with a reference material (Eucalyptus globulus wood) and was subjected to the treatment proposed by Lopez et al. (2015), which involved autohydrolysis and delignification.

Table 1 summarizes the chemical properties of the raw material as determined in this study. As can be seen, the material primarily consisted of cellulose (quantified as α-cellulose), as well as lesser amounts of Klason lignins (determined after quantitative acid hydrolysis), hemicelluloses (23.9%), which was calculated as the combination of xylan (18.8%), araban (2.0%), and acetyl groups (3.1%). This composition is considerably different from the composition of other raw materials used in biorefinery schemes (Pan and Sano 2005; Alfaro et al. 2009, 2010; Caparrós et al. 2007, 2008). Wheat straw and E. globulus contained 38.3% and 20.0% more glucan (α-cellulose), respectively, than tule. César et al. (2015) reported a 15.9% higher α-cellulose content in tule than the content found in this study; however, their hemicellulose content, calculated as the difference between the holocellulose and glucan content, was unusually low (11.5%). Nevertheless, Khider et al. (2012) report only a 4.5% higher α-cellulose content in tule (from Sudan) than the content found in this study and their hemicelluloses content (as pentosans) is unusually high (32.8 %), although analytical methodologies were not the same. The hemicellulose content found in this study was 23.9% lower than the content in sunflower stalks and tagasaste, but was similar to the content in L. diversifolia, wheat straw, and A. donax (27.8%, 20.8%, and 29.7%, respectively). A low hemicellulose content would make the prior extraction of this fraction from the raw material inadvisable with consideration to its valorization (Saha 2003; Fišerová et al. 2012). The ethanol content in the extractives of tule was also different from the content in the other raw materials. However, it was identical with the contents reported by César et al. (2015) and similar to those for A. donax. This difference is typical of grassy materials, e.g., tule or A. donax. The Klason lignin content of tule was similar to the content of the woody species shown in Table 1. Although the ease of delignification of a raw material depends on its lignin content as well as the structural properties of the lignin fraction, tule should be easier to delignify than other woody species (Yedro et al. 2015; Yoo et al. 2020).

This work was undertaken to test the hypothesis that tule is amenable to the valorization of its hemicellulosic fraction via autohydrolysis and that the resulting solid phase will require milder operating conditions than other woody species.

Before the chemical composition of the post-hydrolysis solid and its amenability to pulping were assessed, the authors considered the feasibility of using the solid or the untreated raw material in order to obtain energy. The energy potential of the raw material was assessed by comparing its heating value with the heating value of various other materials. The higher heating value (HHV) of biomass typically ranges from 17598 kJ/kg to 18681 kJ/kg, whereas the HHV of tule has been estimated as 17318 kJ/kg, which is similar to the value of sunflower and only 8.6% lower than L. diversifolia (as shown in Table 1). Therefore, the energy content of tule is amenable to exploitation by combustion with the added advantages of a low environmental impact, a high productivity, and the absence of competition with food-related uses, e.g., starches and cereals. Consequently, tule can be a suitable lignocellulosic biomass for the industrial production of hemicellulosic derivatives, pulp, and paper. For example, the autohydrolysis liquor could be used to obtain furfural, which requires a minimum C5 sugar content of 15% to 20% in the raw material (Mussatto and Mancilha 2007; Patel and Goyal 2011; Cara et al. 2012).

Hydrothermal Treatment

Table 2 shows the values of the independent variables used in accordance with the experimental design and the composition of the resulting solid fractions from autohydrolysis as percentages of the hydrolyzed material. Glucan was the individual polysaccharide that was least affected by autohydrolysis; its content was decreased by 36.7% to 43.6%, i.e., 76.8% and 89.1% of the initial content was preserved. This result is important because a biorefinery process, including paper production, requires the presence of a large amount of sugars in solution but low amounts of cellulose (glucan) degradation in the raw material in order to provide pulp with acceptable properties (Alfaro et al. 2010).

Using a high temperature (165 °C) during the hydrothermal treatment reduced the glucan content of the resulting solids by 40.7% to 43.6%, so 76.8% to 81.4% of the initial content was retained. However, using low and medium temperatures had the opposite effect. At a temperature of 141 and 153 °C, the glucan content was reduced by 36.7% and 40.1%, respectively, and 81.5% to 89.1% of all glucan was retained. The greatest increase in glucan (89.1%) was obtained at 141 °C for 30 min.

As a result of the autohydrolysis process, 19.6% of all biomass in the raw material was extracted into the liquor. The amount of soluble lignins were smaller in most cases (1.83% of the autohydrolysis solid phase). This result suggested substantial dissolution of this fraction due to the autohydrolysis treatment.

Thus, the Klason lignin content in the solid ranged from 21.9% to 29.8%, i.e., 59.1% to 91.2% of the amount present in the raw material, therefore delignifying 8.8% to 40.9% of the Klason lignin content.

The xylose, araban, and acetyl groups contents exhibited substantial changes, i.e., 9.2% to 19.3%, 0.4% to 1.3%, and 1.7% to 3.0%, respectively, relative to the contents in the autohydrolysis solid. Therefore, the autohydrolysis + NaOH-AQ treatment delignified 69.9% to 86.3% of the raw material.

The gross heating value for the hydrolyzed tule was 17598 kJ/kg to 18681 kJ/kg, which is 280 kJ/kg to 1364 kJ/kg (1.6% to 7.9 %) greater than that the gross heating value of the raw material. A high heating value for the autohydrolysis solid phase makes it useful for pulping, the production of chemicals, or burning. The increase in the heating value of tule via autohydrolysis exceeded the heating value of other materials previously autohydrolysed at a temperature range of 180 to 200 °C. Thus, the increase was 0.06% to 2.1% for the reference material (E. globulus), 0.8% to 3.7% for L. diversifolia, 0% to 3.2% for P. fortunei, 0.01% to 1.1% for A. donax, and 0% to 1.7% for C. proliferus (López et al. 2011). Only sunflower stalks clearly exceeded tule in this respect, with an increase of 10.3% to 10.4%. Additionally, the autohydrolysis of tule provided a liquor containing valuable chemicals.

The results for tule and the reference material E. globulus hydrolyzed at 180 °C for 30 min were similar; the autohydrolysis yield for eucalyptus was 84% (Loaiza et al. 2016). The same as tule, the glucan fraction in eucalyptus was barely affected and the hemicelluloses were highly efficiently extracted, even slightly more efficient than tule. Thus, the autohydrolysis treatment recovered 65% to 82% of the hemicellulose content of E. globulus but only 23.3% to 71.6% from tule (Loaiza et al. 2016).

Based on the previous results, and on the reasoning of autohydrolysis, the optimum autohydrolysis conditions for tule were a temperature of 141 °C and a treatment time of 45 min. These conditions ensured a high cellulose (glucan) content in the solid phase and the presence of valorizable hemicellulosic derivatives in the liquor.

Properties of the pulp and paper sheets

As noted in the previous section, the autohydrolysis treatment increased the calorific value of the material via efficiently extracting hemicelluloses. However, the primary target of this work was the delignification of the resulting solid phase under a biorefinery scheme for pulp and paper production. This led the authors to use the autohydrolysis solid obtained under isothermal conditions at a temperature of 141 °C for 45 min as a pulping material.

Table 3 shows the normalized values of the independent variables, the chemical composition of each pulp, and the tensile index of the resulting paper sheets obtained using the proposed experimental design. Each experimental value was the average of 12 results for the tensile index and 5 for the pulp properties. Deviations from the respective means were all less than 10%.

The yield and viscosity of the soda–anthraquinone pulp obtained from autohydrolyzed tule were relatively low (31.1% to 44.5% and 481 cm3/g to 564 cm3/g, respectively). This was a result of subjecting the raw material to two chemical processes in order to remove their lignocellulosic components. However, the prior extraction of hemicelluloses resulted in better kappa number values (20.9 to 39.1). The tensile index of the paper sheets was relatively good but low relative to commercial paper, which is typically obtained from mixed sources. In addition, refining the tule pulp could have led to substantially better properties (Duarte et al. 2011; Sarwar et al. 2012; Hamzeh et al. 2013).

The glucan content of the solid phase resulting from hydrolysis and delignification ranged from 47.6% to 52.2% of the initial amount present in the raw material; the former value was obtained via autohydrolysis with a 20 dry wt% alkali concentration at a temperature of 153 °C for 60 min and the latter was obtained with a 20 dry wt% concentration at temperature of 171 °C for 120 min. These glucan contents were similar to the content in eucalyptus wood (48.7%) processed under similar conditions, i.e., 13 dry wt.% alkali concentration at a temperature of 153 °C for 40 min, but it was lower than the content for the same material subjected to stronger conditions (80.4%), i.e., 17 dry wt% alkali concentration at a temperature of 163 °C for 90 min.

The Klason lignin content ranged from 11.3% with an alkali concentration of 17 dry wt% at a temperature of 163 °C for 120 min to 24.5% with an alkali concentration of 14 dry wt% NaOH at a temperature of 163 °C for 90 min. Therefore, the autohydrolysis treatment delignified 69.9% to 86.3% of the raw material.

The xylan content never exceeded 6%. Loaiza et al. (2016) previously found that autohydrolyzed, delignified E. globulus contained no xylan. The presence of hemicelluloses, even in small amounts, in the solid obtained after the two treatments of tule may have improved some strength-related properties, e.g., tensile index.

Loaiza et al. (2016) obtained a kappa number, viscosity, and tensile index of 29.0, 518 cm³/g, and 18.3 N·m/g, respectively, for tule treated with a 17 dry wt% alkali concentration at a temperature of 163 °C for 90 min. Eucalyptus wood processed under identical conditions had a kappa number, viscosity, and tensile index of 32.9, 769.2 cm3/g, and 15.7 N·m/g, respectively. As shown by these results, the kappa index of the pulp and tensile index of the paper obtained from tule were both higher, but the viscosity of tule pulp was lower.

Based on the above results, tule can be subjected to autohydrolysis in order to destroy its lignin matrix with a view to its subsequent delignification for pulping and the obtainment of a valorizable liquor. The properties of the resulting pulp and paper sheets were similar to those previously obtained from E. globulus (Loaiza et al. 2016), L. diversifolia (Feria et al. 2012), and C. proliferus (Alfaro et al. 2010).

The optimal operating conditions for the autohydrolysis and delignification treatments were established as described below. The discussion that follows is based in part on the last paragraph of the hydrothermal treatment section in regards to the optimum autohydrolysis conditions.

As stated in the experimental design section, the influence the independent variables for autohydrolysis (temperature and time) and pulping (solid yield, glucose content, xylose content, arabinose content, acetyl group content, Klason lignins, soluble lignins, ethanol extractives, kappa number, viscosity, and tensile index) had on each dependent variable was used to construct the mathematical models shown in Table 4. Each value used to establish the equations was the average of three measurements. The differences between the experimental values and those predicted by the equations never exceeded 10%. The fit was always quite good, with R2 greater than 0.90 in all cases.

In order to better envisage the influence of the operational variables on the outcome of the autohydrolysis and pulping processes, the authors constructed the hyperspaces of variation of the dependent variables in the form of response surfaces (as shown in Fig. 1 through Fig. 5). The most influential variable in each case was assigned either of the two extreme values (+1 or -1). A combined plot of the results for the autohydrolysis and pulping processes revealed marked overlap between the spaces and allowed for the identification of the specific operating conditions affording production of cellulose pulp or paper with a given value of some property from the raw material.

Graphical optimization of the autohydrolysis process

Overall, the soda–AQ delignification of tule after autohydrolysis improved the paper properties (tensile index and kappa number). Extracting hemicelluloses has traditionally been assumed to have an adverse impact on the properties of paper sheets obtained from the post-hydrolysis solid; however, in this case, the fact that the solid retained a small portion of the hemicellulose fraction (most was extracted in the autohydrolysis liquor) resulted in improved strength-related properties and an increased kappa number in the produced paper sheets.

Fig. 1. Solid yield and glucan content of the solid as a function of the autohydrolysis temperature and treatment time

Figure 1 shows the percent yield and glucan content of the solid phase obtained from the autohydrolysis process at different temperatures and operation times relative to the raw material. As can be seen, the yield decreased but the glucan content increased as the temperature increased. Due to a combination of both effects, the highest glucan content in the solid (relative to the raw material) was obtained under the optimum autohydrolysis (hemicellulose extraction) conditions. However, these conditions (a temperature of 143 °C and 45 min) resulted in the poor extraction of araban, xylan, and acetyl groups in the liquor, i.e., the highest proportion of xylan was in the solid phase (Fig. 2). However, these conditions allowed a substantial proportion of the hemicelluloses to be extracted from the raw material (25.2%, based on equation 8 shown in Table 4), so they were chosen in order to avoid excessive degradation of the cellulose polymers. In addition, these conditions resulted in the maximal extraction of Klason lignins and soluble lignins in the autohydrolysis liquor (results not shown), which resulted in easier subsequent delignification (Fig. 2).

Fig. 2. Xylan and Klason lignin content in the solid as a function of the autohydrolysis temperature and time

Graphical optimization of the pulp and paper properties

Based on its response surface (not shown), the pulp yield was low as a result of the two-step fractionation of the raw material. Demonstrated by the kappa number (Fig. 3), there was substantial overlap at high active alkali concentrations (20%). This property was much more sensitive to the soda concentration than the other independent variables.

Fig. 3. Variation of the Kappa number as a function of temperature (XT) and operation time (Xt), at two alkali concentrations levels

Overall, the results were much lower than those reported by Loaiza et al. (2016) for the autohydrolysis and subsequent soda pulping of eucalyptus wood (kappa number ranging from 39.1 to 87.9). These results were especially interesting because they suggest that autohydrolysis facilitates subsequent delignification of the raw material. As a consequence, the autohydrolysis treatment leads to lower (more favorable) kappa numbers with lower alkali doses and temperatures, as well as shorter operation times.

The pulp viscosity was most markedly dependent on the autohydrolysis temperature. As shown in Fig. 4, it decreased as the soda concentration decreased, but was barely influenced by the temperature or treatment time, i.e., it was temperature- and time-independent at low soda doses. However, using a high soda dose resulted in minor increases of viscosity.

Gráfico, Gráfico de superficie Descripción generada automáticamente

Fig. 4. Variation of the intrinsic viscosity as a function of temperature (XT) and operation time (Xt), at two alkali concentrations levels

The response surfaces for the pulp samples obtained from the autohydrolysis solid phase are not shown. Instead, Table 4 shows the equations for the glucan, xylan, extractives, and Klason lignin contents. The xylan content of the pulp obtained from the solid was lower than that of the raw material because most of the hemicellulosic derivatives were removed by the autohydrolysis treatment. However, the hemicellulosic derivatives were not completely removed, which resulted in an improved tensile index in the paper sheets.

Figure 5 shows the variation of the tensile index with the alkali concentration and temperature at two different pulping times (normalized values of +1 and –1). As can be seen, the tensile index was as high as 22 N·m/g; hence, the tensile index was similar to the value obtained by Loaiza et al. (2016) using E. globulus, i.e., 22 N·m/g. These values, which were obtained with a soda concentration of approximately 20%, and a medium treatment temperature and time, were consistent with the intrinsic viscosity data. As can be seen from Fig. 5, the viscosity peaked higher than 564 cm3/g at high active alkali concentrations and temperatures, and short treatment times.

Fig. 5. Variation of the tensile index as a function of temperature (XT) and operation time (Xt) at two alkali concentrations levels

Untreated samples of the raw material were also pulped for comparison with the autohydrolyzed material. Both were processed under the strongest conditions in order to confirm whether the pulp and paper properties were influenced by the extent of the hemicellulose extraction.

Table 5 shows such conditions in addition to the physical and chemical properties of the resulting pulp and paper. Although the differences between the pulp from the raw material and the solid phase from autohydrolysis were not significant and never exceeded 10%, the autohydrolysis delignification treatment substantially improved the viscosity and kappa number in addition to providing a valorizable liquor containing 25% to 30% of the hemicellulose fraction found in the raw material. They are results consistent (or better) with those reported by Khider et al. (2012). Under similar operating conditions of temperature (165 to 170 °C), and higher operation time and alkali concentration (210 min and 17% of active alkali as Na2O – 21.93% as NaOH-), pulp yield was between 40.8 and 41.2 %; kappa number was 22.7 and viscosity was between 746 and 836 ml g-1 (at 26 ºSR).

CONCLUSIONS

  1. Tule (Typha domingensis Pers) provided a suitable lignocellulosic biomass for biorefining via an autohydrolysis stage and an alkaline delignification stage. Alkaline cooking the solid residue obtained from the autohydrolysis process with a soda–anthraquinone mixture provided cellulose pulp and paper sheets with similar properties to paper sheets obtained from other raw materials in addition to a valorizable hemicellulose-containing liquor.
  2. The physical properties of the paper sheets obtained from the autohydrolyzed material were acceptable, but somewhat poorer than those provided by other lignocellulosic biomasses, e.g., eucalyptus wood. In any case, these properties can be improved by the mechanical refining of the pulp.
  3. Hydrothermal treatment of tule at temperatures below 141 °C led to an increased pulp yield and stronger paper sheets. The increased hemicellulose content of the raw material makes it especially suitable for the proposed use. In addition, the cellulose content is noticeably less degraded at the temperatures used in the hydrothermal treatment.
  4. The autohydrolysis of tule before alkaline extraction makes it possible to obtain a valuable liquid phase that contains most of the hemicelluloses in the raw material. Also, the gross heating value for the hydrolyzed tule was increased with respect to the raw material.
  5. In summary, tule lends itself readily to valorization for energy production, and also to integral, fractional exploitation via autohydrolysis and soda–anthraquinone delignification, which can additionally bring environmental benefits for sewage water treatment. The optimum autohydrolysis conditions for tule were a temperature of 141 °C and a pretreatment time of 45 min. These conditions ensured a high cellulose (glucan) content in the solid phase and the presence of valorizable hemicellulosic derivatives in the liquor.

ACKNOWLEDGMENTS

This research work was funded by Spain’s Ministry of Economy and Competitiveness (National Programme for Research Aimed at the Challenges of Society (No. CTQ2017-85251-C2-1-R) and Andalusian Regional Government (Project No. RNM 2323-2012) and a post-doctoral fellowship), as well as by the Technological Institute of Ciudad Madero (Tamaulipas State), Mexico, and the National Technological of México (TecNM) with the project [rhh30r (8630)].

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Article submitted: January 19, 2021; Peer review completed: February 28, 2021; Revised version received and accepted: March 13, 2021; Published: March 19, 2021.

DOI: 10.15376/biores.16.2.3328-3347