AbstractCharacterizing the variance of material properties of natural fibers is of growing concern due to a wide range of new engineering applications when utilizing these natural fibers. The aim of this study was to evaluate the variance of the Young’s modulus of sunflower bark by (i) determining its statistical probability distribution, (ii) investigating its relationship with relative humidity, and (iii) characterizing its relationship with the specimen extraction location. To this end, specimens were extracted at three different locations along the stems. They were also preconditioned in three different relative humidity environments. The c2-test was used for hypothesis testing with normal, Weibull, and log-normal distributions. Results show that the Young’s modulus follows a normal distribution. Two-sample t-test results reveal that the Young’s modulus of sunflower stem bark strongly depends on the conditioning’s relative humidity and the specimen’s extraction location; it significantly decreased as the relative humidity increased and significantly increased from the bottom to the top of the stem. The correlation coefficients between the Young’s modulus of different relative humidity values and of specimen extraction locations were determined. The calculation of correlation coefficients shows a linear relation between the Young's modulus and the relative humidity for a given location.