Potent antibacterial activities of solvent extracts (methanol:n-hexane) from the branch, leaf, and root-wood of Salvadora persica were examined against potato phytopathogenic bacteria, namely Pectobacterium carotovorum subsp. carotovorum, Dickeya solani, Ralostonia solanacerum, Enterobacter cloacae, and Bacillus pumilus. The main chemical constituents analyzed by gas chromatography–mass spectrometry (GC/MS) in the branch extracts were N-benzylbenzamide (71.08%), decane (3.17%), stigmasterol (3.17%), 9-desoxo-9-x-acetoxy-3,8,12-tri-O-acetylingol (2.33%), and β-sitosterol (2.15%). The main components in the leaf extracts were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline (28.65%), spiculesporic acid (13.60%), homo-γ-linolenic acid (12.63%), and methyl hexadecanoate (11.01%). The root-wood extracts contained, as primary parts, benzeneacetonitrile (71.47%), 4-aminocarbonyl-5-fluoro-1-α-D-ribofuranosyl-imidazole (10.99%), and benzylisothiocyanate (5.05%). The extracts from the root-wood showed moderate antibacterial activity against the potato bacterial pathogens, which was followed by leaf and branch extracts. The results suggested that S. persica plant extracts could be used as bioagents against potato soft and brown rot bacterial pathogens.
Chemical Composition and Bioactivity of Salvadora persica Extracts against Some Potato Bacterial Pathogens
Mervat EL-Hefny,a Hayssam M. Ali,b,c Nader A. Ashmawy,d and Mohamed Z. M. Salem*,e
Potent antibacterial activities of solvent extracts (methanol:n-hexane) from the branch, leaf, and root-wood of Salvadora persica were examined against potato phytopathogenic bacteria, namelyPectobacterium carotovorum subsp. carotovorum, Dickeya solani, Ralostonia solanacerum,Enterobacter cloacae, and Bacillus pumilus. The main chemical constituents analyzed by gas chromatography–mass spectrometry (GC/MS) in the branch extracts were N-benzylbenzamide (71.08%), decane (3.17%), stigmasterol (3.17%), 9-desoxo-9-x-acetoxy-3,8,12-tri-O-acetylingol (2.33%), and β-sitosterol (2.15%). The main components in the leaf extracts were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline (28.65%), spiculesporic acid (13.60%), homo-γ-linolenic acid (12.63%), and methyl hexadecanoate (11.01%). The root-wood extracts contained, as primary parts, benzeneacetonitrile (71.47%), 4-aminocarbonyl-5-fluoro-1-α-d-ribofuranosyl-imidazole (10.99%), and benzylisothiocyanate (5.05%). The extracts from the root-wood showed moderate antibacterial activity against the potato bacterial pathogens, which was followed by leaf and branch extracts. The results suggested that S. persica plant extracts could be used as bioagents against potato soft and brown rot bacterial pathogens.
Keywords: Salvadora persica; Leaf; Root-Wood; Branch; Antibacterial activity; Chemical composition
Contact information: a: Department of Floriculture, Ornamental Horticulture and Garden Design, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt; b: Botany and Microbiology Department, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi Arabia; c: Timber Trees Research Department, Sabahia Horticulture Research Station, Horticulture Research Institute, Agriculture Research Center, Alexandria, Egypt; d: Department of Plant Pathology, Faculty of Agriculture (El-Shatby), Alexandria University, Alexandria, Egypt; e: Forestry and Wood Technology Department, Faculty of Agriculture (EL-Shatby), Alexandria University, Alexandria, Egypt;
* Corresponding author: email@example.com
Potato is an important vegetable crop in Egypt. Annually, approximately 4,800,000 tons are produced from approximately 178,000 hectares, which makes Egypt the top potato producer in Africa (FAO STAT 2013). Potato plants are subject to numerous pathogens and pests, which cause considerable quantitative and qualitative potato yield losses in Egypt. Such pathogenic problems are caused by bacterial diseases, especially brown rot caused by Ralostonia solanacerum (Yabuuchi et al. 1995) and soft rot and blackleg caused by Pectobacterium carotovorum, Dickeya, Enterobacter, and Bacillus species (Behiry 2013; Salem 2013; Ashmawy et al. 2014).
The first authenticated report of Brown rot disease in Egypt was in the last century (Sabet 1961), and Mickail et al. (1974) made the first survey on the organism. In seed potato production, the contamination of seed tubers with soft rot bacteria (Pérombelon 2002; Toth et al. 2003), is one of the biggest problems, which causes blackleg, rotting of potato stems in the field, and soft rot of tubers during storage (Gardan et al. 2003; Laurila et al. 2008).
Salvadora persica (Miswak), which belongs to family Salvadoraceae, has been used in toothbrushes for the prevention of tooth decay (Arora and Kalia 2013). The leaf extracts act as an antibacterial agent to various oral bacteria (aerobic) with results comparable to known antibiotics (Alali and Al-Lafi 2003).
Several studies have reported that S. persica extracts and seed oil have great medicinal uses in the treatment of nose troubles, gonorrhea, leucoderma, scabies, scurvy, some skin diseases, joint pain and toothaches; it is also used as a laxative and as a general body tonic (Elvin-Lewis et al. 1980; Alali and Al-Lafi 2003; Darmani et al. 2003; Khalessi et al. 2004; Ahmed et al. 2008).
Leaf extracts of S. persica exhibit several pharmacological properties including carminative, antiseptic, antifungal, antibacterial, diuretic, analgesic, anthelmintic, astringent, hypoglycaemic, antiplasmodial, anticaries, antispasmodial, antiscorbutic, and anticonvulsant properties, as well as action against hepatic disorders (Al-Bagieh et al. 1994; Al-Bagieh and Almas 1997; Ali et al.2002; Almas et al. 2005; Saini et al. 2006; Paliwal et al. 2007). Extracts of stems have antiplaque (Chawla 1983) and antimicrobial activities (Almas 2001). Aqueous extracts are more effective than methanol extracts against some pathogenic bacteria (Al-Bayati and Sulaiman 2008); however, Al-Bagieh and Almas (1997) showed that alcoholic extracts have more potent antimicrobial activity than aqueous extracts.
The heterogeneous components extracted from S. persica have been reported to have antimicrobial activities (Akhtar et al. 2011). Pulp and bark extracts show significant differences in their antimicrobial activities (Almas and Al-Bagieh 1999). S. persica extract (20%) is effective as an antifungal and antibacterial agent against Candida albicans and Enterococcus faecalis (Al-Obaida et al. 2010). The diluted acetone extract of dry stems (300 mg/mL) shows good inhibitory activity against C. albicans, C. glabrata, and C. parapsilosis strains with inhibition zones (IZs) that range from 10.33 mm to 15 mm (Noumi et al. 2010).
Volatile oils extracted from the roots and stems of S. persica contain fatty and other organic acid ethyl esters (Abdelrahman et al. 2003). Aqueous extracts of the roots contain chlorine, trimethylamine, and sulphur compounds with antimycotic activity (Al-Otaibi and Angmar 2004). Benzylisothiocyanate is the main component in root oil (Bader et al. 2002), which has good activity against Herpes simplex virus, Streptococcus mutans, and Candida albicans (Al-Bagieh 1992, 1998; Al-Bagieh and Weinberg 1988). β-Sitosterol has been found in the roots of S. persica(Ezmirly et al. 1978). The zone of inhibition against the growth of Staphylococcus aureus ranges from 10.5 mm to 31.5 mm for the leaf extract of S. persica and the combination of tetracycline with the stem extract of S. persica, respectively (Ahmed et al. 2010).
Salvadoricine, an indole alkaloid, has been isolated from S. persica leaves (Malik et al. 1987). Volatile oils from the leaves contain benzyl nitrile, eugenol, thymol, isothymol, eucalyptol, isoterpinolene, and β-caryophyllene (Alali and AL-Lafi 2003). Identified flavanoids and flavanoid glycosides include kaempferol 3-α-l-rhamnosyl-7-β-xylopyranoside, quercetin, and kaempferol (Kamil et al. 2000).
Ethanolic extracts of the stems contain β-sitosterol, stigmasterol, and β-sitosterol-d-glucoside (Arora and Kalia 2013). A sulfated glycoside, salvadoside (sodium 1-O-benzyl-β-d-glucopyranoside-2-sulfate), was isolated from S. persica (Ohtani et al. 1992). Pyrrolidine, pyrrole, and piperidine derivatives have been identified in S. persica sticks (Galletti et al. 1993). Salvadoside and salvadoraside, which are glycoside compounds, have been reported in stem extracts (Kamel et al. 1992). Benzylisothiocyanate, saponins, tannins, resin, trimethylamine, and alkaloid have been isolated from the roots (El-Mostehy et al. 1983). β-Sitosterol, manisic acid, and salvadourea [1,3-bis-(3-methoxy-benzyl)-urea] were isolated from the root by Ray et al.(1975). 2-Furancarboxaldehyde-5-(hydroxymethyl), furan-2-carboxylic acid-3-methyl- trimethylsilyl ester, and d-erythro-pentofuranose-2-deoxy-1,3,5-tris-O-(trimethylsilyl) were identified in root methanol extracts; these components exhibit antioxidant activities (Mohamed and Khan 2013). Stem essential oils include 1,8-cineole (eucalyptol), α-caryophellene, β-pinene, and 9-epi-(E)-caryophellene as the major components (Alali et al. 2004).
Most of the studies related to the bioactivity of extracts from S. persica have focused on the extractives’ effectiveness as a natural tool for dental cleaning and as a natural analgesic for toothache, as well as their effect on various aspects of oral health (Alali and Al-Lafi 2003; Balto et al. 2012; Halawany 2012; Chaurasia et al. 2013).
The antibacterial activities of extracts from several plants against bacterial potato pathogens have been assessed, and quite satisfactory results have been observed (Salem 2013; Ashmawy et al.2014). The agricultural companies in the Mediterranean countries are focused on the commercial production of known aromatic herbs such as mint and basil (Edris et al. 2003) and neglecting the utilization of trees and shrubs, which may provide new sources of medical and agricultural applications (Bakkali et al. 2008; Abdel-Megeed et al. 2013; Salem et al. 2013, 2014a,b,c). So there is motivation to search for new and renewable sources for natural products that are useful against phytopathogenic bacteria and fungi (Salem et al. 2016a,b).
To date, there are no reports on the bioactivity of extracts from S. persica against the growth of pathogenic bacteria that attack plants. This study evaluated the antibacterial activity of extracts that analyzed by gas chromatography–mass spectrometry (GC/MS) from the leaves, branches, and root-wood of S. persica against the growth of some pathogenic bacteria.
Plant Materials and Reagents
Leaves, branches, and root-wood of Salvadora persica were collected in May 2016 from the Jazan Region located on the southwestern part of the Kingdom of Saudi Arabia. The plant was identified by the Botany and Microbiology Department of the College of Science at King Saud University. The samples were delivered to the Faculty of Agriculture at Alexandria University by Dr. Hayssam M. Ali on June 2016. Extractions were performed at Alexandria University on the various S. persica components, and the antibacterial activity of extractives was assessed. The plant was authenticated with the voucher number Zidan0043. Methanol, dimethylsulfoxide (DMSO) and n-hexane solvents were bought from Sigma Aldrich (Cairo, Egypt).
About 100 air-dried g of powdered leaf, branch, and root-wood were separately extracted by soaking in a mixture of methanol:n-hexane (1:1 v/v) for one week. The extraction process was repeated three times in the week until exhaustion, where every filtration was done after two days. The combined extract from each plant part was concentrated using a rotary-evaporator at 45 °C. The concentrated extracts were stored for one week at 4 °C until further analysis. The extract weights from leaf, branch, and root-wood components were 6.24, 5.17, and 8.55 g, respectively. Each extract was prepared in the concentrations of (1000, 500, 250, 125, 64, and 32 µg/mL), by diluting the extract in 10% DMSO.
Antibacterial Activity Assay
The antibacterial activities of leaf, branch, and root-wood extracts from S. persica were evaluated using the disc diffusion method of Bauer et al. (1966) against the growth of selected phytopathogenic bacteria: Pectobacterium carotovorum subsp. carotovorum ippbc038, Dickeya solani, Ralostonia solanacerum, Enterobacter cloacae, and Bacillus pumilus. These bacterial strains have been associated with blackleg and soft rot disease of potatoes; also, these bacteria can completely destroy potato plantations, as well as cause brown rot in potatoes after post-harvest. The discs were impregnated with 20 µL of each of the concentrated extract (leaf, branch, and root-wood extracts). Mueller Hinton Agar (MHA) media in sterile Petri dishes were spread with a fresh 24-h-old bacterial suspension (1.0 x 105 CFU/mL) and sterile discs (Whatman filter paper no. 1) with 4 mm diameter and were stacked over the inoculated media surface. Three measurements of the inhibition zones around the discs were recorded in millimeters using a ruler.
The bacterial strains were supplied by the Department of Plant Pathology of the Faculty of Agriculture (El-Shatby) at Alexandria University (Alexandria, Egypt). Control discs with negative (DMSO) and positive (gentamicin 20 μg/disc) were performed, and all tests were performed in triplicate.
GC/MS Analyses of Extracts
The chemical compositions of the extracts were analyzed using a Trace GC Ultra-ISQ Mass Spectrometer (Thermo Scientific, Austin, TX, USA) with a direct capillary column TG-5MS (30 m × 0.25 mm × 0.25 µm film thickness) apparatus. The GC/MS was located at the Atomic and Molecular Physics Unit of the Experimental Nuclear Physics Department at the Nuclear Research Centre of the Egyptian Atomic Energy Authority (Inshas, Cairo, Egypt). The column oven temperature was initially held at 120 °C and then increased by 5 °C∙min-1 to 200 °C, which was held for 2 min, then increased to 280 °C (10 °C∙min-1). Temperatures of the injector and detector (MS transfer line) were kept at 250 °C. Helium, which was the carrier gas, was kept in constant flow rate of 1 mL∙min-1. The solvent delay was 2 min, and diluted samples of 1 µL were injected automatically using an Auto-sampler AS3000 coupled with the GC unit in the split mode. EI mass spectra were collected at 70 eV ionization voltages over the m/z range of 40 to 550 in full scan mode. The ion source and transfer line temperatures were set at 200 and 250 °C, respectively. The components were identified by comparison of their retention times and mass spectra with those of the WILEY 09 and NIST 11 mass spectral database (Davies 1990).
The values of the antibacterial activity are presented as mean of three replicates. Analysis of variance (ANOVA) was used to evaluate the significant difference among various treatments with the criterion of p = 0.05. The statistical analysis was performed using SAS software version 8.2 (2001).
RESULTS AND DISCUSSION
As shown in Table 1, the root-wood extracts exhibited good bioactivity against the growth of Ralostonia solanacerum at 1000, 500, 250, 125, and 64 µg/mL levels with inhibition zone (IZ) values of 12.00, 11.33, 11.00, 11.00, and 11.00 mm, respectively. Furthermore, good bioactivity activity was observed with leaf extracts at 1000 µg/mL with an IZ value of 11.66 mm, and root-wood at 500 µg/mL (IZ value of 11.33 mm). The highest IZ values of the extracts was found against the growth of Enterobacter cloacae, with 11.00 mm for root-wood extracts at 1000, 500, and 250 µg/mL levels, followed by leaf extracts with 10.00 mm at the same concentration levels.
Table 1. Antibacterial Activity of Extracts from S. persica Leaf, Branch, and Root-Wood
Values are mean of three replicates. Means with the same letter within the same column are not significantly difference according to LSD0.05. *Positive control; discs of 10 μg Gentamicin. BrSB: S. persica branch; LefSB: S. persica leaf; RSB: S. persica root-wood
For Bacillus pumilus, the most active extract was found from the root-wood at 1000 µg/mL with IZ value of 12.00 mm, which was followed by branch extracts with 11.33 mm at 1000 µg/mL. In addition, good activity (11.00 IZ value) was observed from extracts of branch (500 µg/mL), leaf (1000 µg/mL), and root-wood (500 µg/mL). Root-wood extracts showed good activity against Pectobacterium carotovorum with IZ value of 14.66 mm at 1000 µg/mL, followed by 12.66 mm at 500 µg/mL. Extracts of leaf showed some activity at 1000 µg/mL (11.00 mm), 500 µg/mL (10.00 mm), and 250 µg/mL (10.00 mm). Branch extracts showed activity against the growth of Dickeya solani at 1000 µg/mL with an IZ value of 10.33 mm, followed by leaf extracts at 1000 µg/mL (IZ 10.00 mm), and root-wood extracts at 1000 µg/mL (IZ 10.00 mm) and at 500 µg/mL (IZ 10.00 mm). Based on the these results, the root-wood extracts from S. persica had better antibacterial activity against the growth of the studied bacteria compared to leaf and branch extracts. Overall, the IZ values presented from the extracts are lower than those reported from the antibiotic used (Gentamicin).
All over the world, many trials have been done to control the diseases of potatoes without promising control. Some success has been reported with chemical control of brown rot (Murakoshi and Takahashi 1984), soil fumigants (Weingartner and Shumaker 1988), resistant varieties (Fock et al. 2001; Lopez and Biosca 2004), and antibiotics (Habashy et al. 1993). Additionally, chemical control (pesticides) with its residues has been reported to have hazardous effects in Europe and Egypt (Sylvander and Le Floc’h-Wadel 2000; Parrott and Kalibwani 2004).
Table 2. Identified Chemical Components of Methanol:n-Hexane Branch Extracts of S. persica
Therefore, some studies have focused on using natural extracts for controlling potato disease. For example, Salem (2013) found that the bark extracts of Delonix regia and Erythrina humeana exhibited moderate antibacterial activity against the growth of potato soft rot bacteria D. dianthicola, P. carotovorum subsp. wasabiae, P. carotovorum subsp. carotovorum, P. carotovorum subsp. atrosepticum, and D. chrysanthemi. Additionally, the extracts from Tecoma stans leaves and branches also exhibited good activity compared with the extracts from Callistemon viminalis against the same bacteria strains. Salem et al. (2016a) found that the wood and bark extracts from Picea abies and Larix decidua showed moderate activity against the growth of P. atrosepticum, P. carotovorum subsp. carotovorum and D. solani. Stenotrophomonas maltophilia, isolated from the rhizosphere of eggplant cultivated in the Nile Delta of Egypt, was found to be potential biocontrol agent of R. solanacerum (Messiha et al. 2007).
Earlier studies of the antimicrobial effects of extracts from S. persica showed that the methanol extracts were less active than the aqueous extracts for the inhibition of S. aureus, Streptococcus mutans, Streptococcus pyogenes, E. faecalis, Lactobacillus acidophilus, Pseudomonas aeruginosa, and Candida albicans growth (Al-Bayati and Sulaiman 2008). The strong antimicrobial effects of extracts from S. persica against the growth of bacteria, fungi, and viruses have been attributed to volatile active compounds (Ali et al. 2002; Al-Mohaya et al. 2002; Hamza et al. 2006; Sofrata et al. 2008).
Chemical Constituents of Extracts
The methanol:n-hexane branch extracts of S. persica were analyzed by GC/MS, which identified 20 components (Table 2). The main chemical constituents in the extracts were: N-benzylbenzamide (71.08%), decane (3.17%), stigmasterol (3.17%), 9-desoxo-9-x-acetoxy-3,8,12-tri-O-acetylingol (2.33%), and β-sitosterol (2.15%).
GC/MS analysis of leaf extracts, which identified 23 components (Table 3), showed the presence of the following main components: 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline (28.65%), spiculesporic acid (13.60%), homo-γ-linolenic acid (12.63%), methyl hexadecanoate (11.01%), and hexadecanoic acid (7.30%).
The GC/MS analysis of methanol:n-hexane extracts of the root-wood of S. persica (Table 4) identified six (6) main components: benzeneacetonitrile (71.47%), 4-aminocarbonyl-5-fluoro-1-α-d-ribofuranosyl-imidazole (10.99%), benzylisothiocyanate (5.05%), 2,2-dimethoxybutane (4.83%), N-benzylidenebenzylamine (4.36%), and 3-methyl-1-(phenylmethyl)azetidine (3.3%). Previously, most identified compounds were related to alkloidal constituents (i.e.,benzeneacetonitrile, and 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline) (El-Mostehy et al.1983; Malik et al. 1987; Bhandari 1990; Galletti et al. 1993; Darout et al. 2000; Alali and Al-Lafi 2003). In addition, carbohydrates, steroids, alkaloids, saponins, tannins, triterpenes, glycosides, mucilage, fats and oils have been reported from leaves and stems extracts of S. oleoides (Arora et al. 2014).
Phytol and n-hexadecanoic acid were the main components in leaf and stem extracts of Salvadora oleoides (Samejo et al. 2012). Butanediamide, N,N-bis(phenylmethyl)-2(S)-hydroxy-butanediamide, N-benzyl-2-phenylacetamide, N-benzylbenzamide, and benzylurea were isolated from the stems of S. persica (Khalil 2006). Fatty acids esters, such as oleic, linolic, and stearic acid, as well as some terpenoids, were investigated in the volatile compounds in S. persica crude extract (Abdelrahman et al. 2003). There were other volatile components, such as benzyl nitrile, eugenol, thymol, isothymol, eucalyptol, isoterpinolene, and β-caryophyllene (Alali and Al-Lafi 2003). The aqueous extracts from roots and stems had antimicrobial components that were anionic, such as sulfate, chloride, thiocynate, and nitrate (Darout et al. 2000). Root extracts contain salvadourea (Ray et al. 1975) and benzylisothiocynate (Al-Bagieh 1990). Benzyl isothiocyanate was found to be highly active against Gram- Negative bacteria (Bader et al. 2002; Sofrata et al. 2011).
Table 3. Identified Chemical Components of Methanol:n-Hexane Leaf Extracts of S. persica
Table 4. Identified Chemical Components of Methanol:n-Hexane Root-Wood Extracts from S. persica
While the preliminary results from this study appear promising, further studies are recommended to justify the phytochemical application of S. persica extracts in the field as a natural pesticide for controlling phytopathogenic bacteria.
- Leaf, branch, and root-wood extracts of Salvadora persica exhibited good antibacterial activity against the bacterial pathogens Pectobacterium carotovorum subsp. carotovorum,Dickeya solani, Ralostonia solanacerum, Enterobacter cloacae, and Bacillus pumilus, which attack potato plants.
- The main chemical constituents in the branch extracts were N-benzylbenzamide, decane, stigmasterol, 9-desoxo-9-x-acetoxy-3,8,12-tri-O-acetylingol, and β-sitosterol. Additionally, the main constituents in the leaf extracts were 2,6-dimethyl-N-(2-methyl-α-phenylbenzyl)aniline, spiculesporic acid, homo-γ-linolenic acid, and methyl hexadecanoate. Likewise, the main constituents in the root-wood extracts were benzeneacetonitrile, 4-aminocarbonyl-5-fluoro-1-α-d-ribofuranosyl-imidazole, and benzylisothiocyanate.
- The root-wood extracts from S. persica had better antibacterial activity against the growth of the studied potato bacterial pathogens compared to those reported from leaf and branch extracts, but all the inhibition zones were lower than the values reported from the antibiotic used (Gentamicin).
This project was supported by the King Saud University, Deanship of Scientific Research, College of Science Research Center.
Abdel-Megeed, A., Salem, M. Z. M., Ali, H. M., and Gohar, Y. M. (2013). “Brachychiton diversifolius as a source of natural products: Antibacterial and antioxidant evaluation of the extracts of wood branches,” J. Pure Appl. Microbiol. 7(3), 1843-1850.
Abdelrahman, H. F., Skaug, N., Whyatt, A. M., and Francis, G. W. (2003). “Volatile compounds in crude Salvadora persica extracts,” Pharm. Boil. 41(6), 399-404. DOI: 10.1076/phbi.41.6.399.17826
Ahmed, Z., Khan, S. S., Khan, M., Tanveer, A., and Lone, Z. A. (2010). “Synergistic effect of Salvadora persica extracts, tetracycline and penicillin against Staphylococcus aureus,” African J. Basic Appl. Sci. 2(1-2), 25-29.
Ahmed, S., Soaad, E., Essawy, E., Mohamed, E. I., and Ewald, S. (2008). “Preliminary phytochemical and propagation trial with Salvadora persica L,” Agric. For Res. 1, 135-138.
Akhtar, J., Siddique, K., Bi, S., and Mujeeb, M. (2011). “A review on phytochemical and pharmacological investigations of miswak (Salvadora persica Linn.),” J. Pharm. Bio. Allied Sci.3(1), 113-117. DOI: 10.4103/0975-7406.76488
Alali, F., and Al-Lafi, T. (2003). “GC-MS analysis and bioactivity testing of the volatile oil from the leaves of the toothbrush tree Salvadora persica L.,” Nat. Prod. Res. 17(3), 189-94. DOI: 10.1080/1057563021000040790
Alali, F., Hudaib, M., Aburjai, T., Khairallah, K., and Al-Hadidi, N. (2004). “GC-MS analysis and antimicrobial activity of the essential oil from the stem of the Jordanian toothbrush tree Salvadora persica,” Pharm. Biol. 42(8), 577-80. DOI: 10.1080/13880200490901834
Al-Bagieh, N. H., and Almas, K. (1997). “In vitro antimicrobial effects of aqueous and alcohol extracts of Miswak,” Cairo Dental J. 13, 221-224.
Al-Bagieh, N. H., Idowu, A., and Salako, N. O. (1994). “Effect of aqueous extract of miswak on the in vitro growth of Candida albicans,” Microbios 80(323), 107-13.
Al-Bagieh, N. H. (1990). “Antiherpes simplex c-virus type 1 activity of benzyisothicyanate,” Biomed. Lett. 47, 67-70.
Al-Bagieh, N. H. (1992). “Antiherpes simplex virus type 1 activity of benzylisothiocyanate,” Biomed. Lett. 80, 107-113.
Al-Bagieh, N. H. (1998). “Effect of benzylisothiocyanate on the growth and acid production of Candida albicans,” Biomed. Lett. 58, 139-145.
Al-Bagieh, N. H., and Weinberg, E. D. (1988). “Benzylisothiocyanate: A possible agent for controlling dental caries,” Microbios Lett. 39, 143-151.
Al-Bayati, F., and Sulaiman, K. (2008). “In vitro antimicrobial activity of Salvadora persica L. extracts against some isolated oral pathogens in Iraq,” Turk. J. Biol. 32(1), 57-62.
Ali, H., Konig, G. M., Khalid, S. A., Wright, A. D., and Kaminsky, R. (2002). “Evaluation of selected Sudanese medicinal plants for their in vitro activity against hemoflagellates, selected bacteria, HIV-1-RT and tyrosine kinase inhibitory, and for cytotoxicity,” J. Ethnopharmacol.83(3), 219-228. DOI: 10.1016/S0378-8741(02)00245-3
Almas, K., Skaug, N., and Ahmad, I. (2005). “In vitro antimicrobial comparison of Miswak extract with commercially available non-alcohol mouthrinses,” Int. J. Dent. Hyg. 3(1), 18-24. DOI: 10.1111/j.1601-5037.2004.00111.x
Almas, K. (2001). “The antimicrobial effects of seven different types of Asian chewing sticks,” Odontostomatol Trop. 24(96), 17-20.
Almas, K., and Al-Bagieh, N. (1999). “The antimicrobial effects of bark and pulp extracts of miswak, Salvadora persica,” Biomed. Lett. 60, 71-75.
Al-Mohaya, M. A., Darwazeh, A., and Al- Khudair, W. (2002). “Oral fungal colonization and oral candidiasis in renal transplant patients: The relationship to Miswak use,” Oral Surg. Oral Med. Oral Pathol. Oral Radiol. Endod. 93(4), 455-460. DOI: 10.1067/moe.2002.121992
Al-Obaida, M. I., Al-Essa, M. A., Asiri, A. A., and Al-Rahla, A. A. (2010). “Effectiveness of a 20% miswak extract against a mixture of Candida albicans and Enterococcus faecalis,” Saudi Med. J. 31(6), 640-643.
Al-Otaibi, M., and Angmar, B. (2004). “Oral hygiene habits and oral health awareness among urban Saudi Arabians,” Oral Heal. Prev. Dent. 2(4), 389-96.
Arora, M., and Kalia, A. N. (2013). “Isolation and characterization of stigmasterol and β-sitosterol-d-glycoside from ethanolic extract of the stems of Salvadora persica linn.,” Int. J. Pharm. Sci. 5(1), 245-249.
Arora, M., Siddiqui, A. A., Paliwal, S., and Sood, P. (2014). “A phyto-pharmacological overview on Salvadora oleoides Decne,” Indian J. Nat. Prod. Res. 5(3), 209-214.
Ashmawy, N. A., Behiry, S. I., Ali, H. M., and Salem, M. Z. M. (2014). “Evaluation of Tecoma stans and Callistemon viminalis extracts against potato soft rot bacteria in vitro,” J. Pure Appl. Microbiol. 8(Special Ed. 2), 667-673.
Bader, A., Flamini, G., Luigi, P., and Morelli, I. (2002). “The composition of the root oil of Salvadora persica L.,” J. Essen. Oil Res. 14(2), 128-129. DOI: 10.1080/10412905.2002.9699795
Bakkali, F., Averbeck, S., Averbeck, D., and Idaomar, M. (2008). “Biological effects of essential oils – A review,” Food Chem. Toxicol. 46(2), 446-475. DOI: 10.1016/j.fct.2007.09.106
Balto, H., Ghandourah, B., and Al-Sulaiman, H. (2012). “The efficacy of Salvadora persica extract in the elimination of the intracanal smear layer: A SEM study,” Saudi Dent. J. 24 (2), 71-77. DOI:10.1016/j.sdentj.2012.01.002
Bauer, A.W., Kirby, W. M., Sherris, J. C., and Turck, M. (1966). “Antibiotic susceptibility testing by a standardized single disk method,” Am. J. Clin. Path. 45(4), 493-496.
Behiry, S. I. (2013). Molecular and Pathological Studies on Potato Bacterial Soft Rot Disease, Ph.D. Dissertation, Alexandria University, Alexandria, Egypt.
Bhandari, M. M. (1990). Flora of the Indian Desert (1st Ed.), Dhrti Printers, New Delhi, India.
Chaurasia, A., Patil, R., and Nagar, A. (2013). “Miswak in oral cavity – An update,” J. Oral Boil. Craniofacial Res. 3(2), 98-101. DOI: 10.1016/j.jobcr.2012.09.004
Chawla, H. S. (1983). “A new natural source for topical fluoride,” J. Indian Dent. Assoc. 55(10), 419-422.
Darmani, H., Al-Hiyasat, A. S., Elbetieha, A. M., and Alkofahi, A. (2003). “The effect of an extract of Salvadora persica (meswak, chewing stick) on fertility of male and female mice,” Phytomed. 10(1), 63-65. http://dx.doi.org/10.1078/094471103321648683
Darout, I. A., Christy, A. A., Skaug, N., and Egeberg, K. P. (2000). “Identification and quantification of some potentially antimicrobial anionic component in miswak extract,” Indian J. Pharmacol. 32(1), 11-14.
Davies, N. W. (1990). “Gas chromatographic retention indices of monoterpenes and sesquiterpenes on methyl silicone and Carbowax 20 M phases,” J. Chromatogr. A 503(2015), 1-24.
Edris, A. E., Shalaby, A. S., Fadel, H. M., and Abdel-Wahab, M. A. (2003). “Evaluation of a chemotype of spearmint (Mentha spicata L.) grown in Siwa Oasis, Egypt,” Eur. Food Res. Technol. 218(1),74-78. DOI: 10.1007/s00217-003-0802-4
El-Mostehy, M. R., Al-Jassem, A. A., Al-Yassin, I. A., Al-Gindy, A. R., and Shoukry, E. (1983). “Miswak as an oral health device. Preliminary chemical and clinical evaluation,” Hamdard. 26(4), 41-50.
Elvin-Lewis, M., Hall, J. B., and Adu-uta, M. (1980). “The dental health of chewing stick users of southern Ghana, preliminary finding,” J. Prev. Dent. 6, 151-159.
Ezmirly, S. T., Cheng, J. C., and Wilson, S. R. (1978). “Saudi Arabian medicinal plants: Salvadora persica,” Planta Med. 35(2), 191-192. DOI: 10.1055/s-0028-1097205
FAO, STAT (2013). “FAOSTAT database,” (http://faostat.fao.org), Food and Agriculture Organization of the United Nations, Rome, Italy.
Fock, I., Collonnier, C., Luisetti, J., Purwito, A., Souvannavong, V., Vedel, F., Servaes, A., Ambroise, A., Kodja, H., Ducreux, G., and Sihachakr, D. (2001). “Use of Solanum stenotomum for introduction of resistance to bacterial wilt in somatic hybrids of potato,” Plant Physiol. Biochem. 39(10), 899-908. http://dx.doi.org/10.1016/S0981-9428(01)01307-9
Galletti, G., Chiavari, G., and Kahie, Y. (1993). “Pyrolysis/gas chromatography/ion-trap mass spectrometry of the ‘tooth brush’ tree (Salvadora persica L.),” Rapid Commun. Mass Spectrom.7(7), 651-655. DOI: 10.1002/rcm.1290070719
Gardan, L., Gouy, C., Christen, R., and Samson, R. (2003). “Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov.,” Int. J. Syst. Evol. Microbiol. 53(Pt 2), 381-391. DOI: 10.1099/ijs.0.02423-0
Habashy, W. H. S., Fawzi, F. G., El-Huseiny, T. M., and Neweigy, N. A. (1993). “Bacterial wilt of potatoes. II. Sensitivity of the pathogen to antibiotics and pathogenesis by streptomycin-resistant mutants,” Egyptian Journal of Agricultural Research 71, 401-412.
Halawany, H. S. (2012). “A review on miswak (Salvadora persica) and its effect on various aspects of oral health,” Saudi Dent. J. 24(2), 63-69. DOI: 10.1016/j.sdentj.2011.12.004
Hamza, O. J., Bout-van, C. J., Matee, M. I., Moshi, M. J., Mikx, F. H., Selemani, H. O., Mbwambo, Z. H., Vander, A. J., and Verweij, P. E. (2006). “Antifungal activity of some Tanzanian plants used traditionally for the treatment of fungal infections,” J. Ethnopharmacol.108(1), 124-132. DOI:10.1016/j.jep.2006.04.026
Kamel, M., Ohtani, K., and Assaf, M. (1992). “Lignan glycosides from stems of Salvadora persica,” Phytochemistry 31(7), 2469-2471. DOI: 10.1016/0031-9422(92)83301-E
Kamil, M., Ahmad, F., Jayaraj, A. F., Gunasekhar, C., Thomas, S., Habibullah, M., and Chan, K. (2000). “Isolation and identification of a flavonol glycoside using high speed counter current chromatographic technique from the leaves of Salvadora persica,” Pak. J. Sci. Ind. Res. 43(4), 255-257.
Khalessi, A. M., Pack, A. R. C., Thomson, W. M., and Tompkins, G. R. (2004). “An in vivo study of the plaque control efficacy of Persica TM: A commercially available herbal mouthwash containing extracts of Salvadora persica,” Int. Dent. J. 54(5), 279-283. DOI: 10.1111/j.1875-595X.2004.tb00294.x
Khalil, A. T. (2006). “Benzylamides from Salvadora persica,” Arch. Pharm. Res. 29(11), 952-956. DOI: 10.1007/BF02969277
Laurila, J., Ahola, V., Lehtinen, A., Joutsjoki, T., Hannukkala, A., Rahkonen, A., and Pirhonen, M. (2008). “Characterization of Dickeya strains isolated from potato and river water samples in Finland,” Eur. J. Plant. Pathol. 122(2), 213-225. DOI: 10.1007/s10658-008-9274-5
Lopez, M. M., and Biosca, E. G. (2004). “Potato bacterial wilt management: New prospects for an old problem,” in: Bacterial Wilt Disease and the Ralostonia Species Complex, C. Allen, P. Prior, and A. C. Hayward (eds.), APS Press, St. Paul, Minnesota, USA, pp. 205-224
Malik, S., Ahmad, S. S., Haider, S. I., and Muzaffar, A. (1987). “Salvadoricine, a new alkaloid from the leaves of Salvadora persica,” Tetrahedron Lett. 28(2), 163-164. DOI: 10.1016/S0040-4039(00)95675-2
Messiha, N. A. S., van Diepeningen, A. D., Farag, N. S., Abdallah, S. A., Janse, J. D., and van Bruggen, A. H. C. (2007). “Stenotrophomonas maltophilia: A new potential biocontrol agent of Ralostonia solanacerum, causal agent of potato brown rot,” Eur. J. Plant Pathol. 118(3), 211-225. DOI: 10.1007/s10658-007-9136-6
Mickail, K. Y., Bishay, F., Farag, N. S., and Tawfik, A. E. (1974). “Evaluation of bacterial wilt disease in A.R.E. during the years from 1967–1968 to 1971–1972,” Agri. Res. Rev. (Cairo) 52, 89-94.
Mohamed, S.A., and Khan, J. A. (2013). “Antioxidant capacity of chewing stick miswak Salvadora persica,” BMC Complem. Altern. Med. 13(40). DOI: 10.1186/1472-6882-13-40
Murakoshi, S., and Takahashi, M. (1984). “Trials of some control of tomato wilt caused by Pseudomonas solanacerum,” Bulletin of the Kanagawa Horticultural Experiment Station 31, 50-56.
Noumi, E., Snoussi, M., Hajlaoui, H., Valentin, E., and Bakhrouf, A. (2010). “Antifungal properties of Salvadora persica and Juglans regia L. extracts against oral Candida strains,” Eur. J. Clin. Microbiol. Infect. Dis. 29(1), 81-88. DOI: 10.1007/s10096-009-0824-3
Ohtani, K., Kasai, R., Yamasaki, K., Tanaka, O., Kamel, M. S., Assaf, M. H., El-Shanawani, M. A., and Ali. A. A. (1992). “Lignan glycoside from stems of Salvodora persica L,” Phytochemistry31(7), 2469-2471. DOI: 10.1016/0031-9422(92)83301-E
Paliwal, S., Chauhan, R., Siddiqui, A. A., Paliwal, S., and Sharma, J. (2007). “Evaluation of antifungal activity of Salvadora persica Linn. leaves,” Nat. Prod. Rad. 6(5), 372-374.
Parrott, N., and Kalibwani, F. (2004). “Organic agriculture in the continents, Africa,” in: The World of Organic Agriculture Statistics and Emerging Trends, H. Willer and M. Yussefi (eds.), pp. 55-68.
Pérombelon, M. C. M. (2002). “Potato diseases caused by soft rot erwinias: An overview of pathogenesis,” Plant Pathol. 51(1), 1-12. DOI: 10.1046/j.0032-0862.2001.Shorttitle.doc.x
Ray, A. B., Chand, L., and Dutta, S. C. (1975). “Salvadoure, a new urea derivative from Salvadora persica,” Chem. Ind. 12, 517-518.
Sabet, K. A. (1961). “The occurrence of bacterial wilt of potatoes caused by Pseudomonas solanacerum (E.F. Smith) in Egypt,” Cairo, General Organisation for Govt. Print. Offices, 1961.
Salem, M. Z. M. (2013). “Evaluation of the antibacterial and antioxidant activities of stem bark extracts of Delonix regia and Erythrina humeana grown in Egypt,” J. Forest Prod. Ind. 2(2), 48-52.
Salem, M. Z. M., Ali, H. M., El-Shanhorey, N. A., and Abdel-Megeed, A. (2013). “Evaluation of extracts and essential oil from Callistemon viminalis leaves: Antibacterial and antioxidant activities, total phenolic and flavonoid contents,” Asian Pac. J. Trop. Med., 6(10):785–791.http://dx.doi.org/10.1016/S1995-7645(13)60139-X
Salem, M. Z. M., Ali, H. M., and Mohamed, N. H. (2014a). “Evaluation of extracts from different parts of some tree species against the growth of some human bacterial pathogens,” J. Pure Appl. Microbio. 8(Spl. Edn. 1), 149-154.
Salem, M. Z. M., Khamis, M. H., El-Shanhorey, N. A., Al-Muwayhi, M. A., Okla, M. K., and Ali, H. M. (2014b). “Evaluation of extracts from leaves of Brachychiton diversifolius R.Br against thegrowth of some clinical pathogens,” J. Pure Appl. Microbio. 8(Spl. Edn. 1), 105-109.
Salem, M. Z. M., Abdel-Megeed, A., and Ali, H. M. (2014c). “Stem wood and bark extracts of Delonix regia (Boj. Ex. Hook): Chemical analysis, antibacterial, antifungal, and antioxidant properties,” BioResources 9(2), 2382-2395.
Salem, M. Z. M., Elansary, H. O., Elkelish, A. A., Zeidler, A., Ali, H. M., EL-Hefny, M., and Yessoufou, K. (2016a). “In vitro bioactivity and antimicrobial activity of Picea abies and Larix decidua wood and bark extracts,” BioResources 11(4), 9421-9437. DOI: 10.15376/biores.11.4.9421-9437
Salem, M. Z. M., Zayed, M. Z., Ali, H. M., and Abd El-Kareem, M. S. M. (2016b). “Chemical composition, antioxidant and antibacterial activities of extracts from Schinus molle L. wood branch growing in Egypt,” J. Wood Sci. 62(6), 548-561. DOI: 10.1007/s10086-016-1583-2
Saini, S., Yadav, J. P., and Kalia A. N. (2006). “Hypoglycemic activity of Salvadora persica and S. oleoides in diabetic albino rats,” J. Sci. Pharm. 7 (1), 5-12.
Samejo, M. Q., Memon, S., Bhanger, M. I., and Khan, K. M. (2012). “Chemical constituents of essential oil of Salvadora oleoides,” J. Pharm. Res. 5(4), 2366-2367.
SAS, (2001). Users Guide: Statistics (Release 8.02), SAS Inst. Inc., Cary, NC, USA, 2001.
Sofrata, A. H., Claesson, R. L., Lingstram, P. K., and Gustafsson, A. K. (2008). “Strong antibacterial effect of miswak against oral microorganisms associated with periodontitis and caries,” J. Periodontol. 79(8), 1474-1479. DOI: 10.1902/jop.2008.070506
Sofrata, A., Santangelo, E. M., Azeem, M., Borg-Karlson, A.-K., Gustafsson, A., and Pütsep, K. (2011). “Benzyl isothiocyanate, a major component from the roots of Salvadora Persica is highly active against gram-negative bacteria,” PLoS ONE 6(8), e23045. DOI: 10.1371/journal.pone.0023045
Sylvander, B., and Le Floc’h-Wadel, A., (2000). “Consumer demand and production of organics in the EU,” AgBioForum 3(2&3), 97-106
Toth, I. K., Bell, K. S., Holeva, M. C., and Birch, P. R. J. (2003). “Soft-rot Erwina: From genes to genomes,” Mol. Plant Pathol. 4(1), 17-30. DOI: 10.1046/j.1364-3703.2003.00149.x
Weingartner, D. P., and Shumaker, J. R. (1988). “In row injection of metham sodium and other soil fumigants for control of nematodes and soil borne potato diseases in Florida,” 72nd Annual Meeting of The Potato Association of America. American Potato Journal, Fort Collins, Colorado, USA, 65: 504.
Yabuuchi, E. Y., Kosaku, I., Yano, I., Hotta, H., and Nishiuchi, Y. (1995). “Transfer of two Burkholderia and an Alcaligenes species to Ralostonia gen. Nov.,” Microbiol. Immunol. 39(11), 897-904. DOI: 10.1111/j.1348-0421.1995.tb03275.x
Article submitted: October 21, 2016; Peer review completed: January 11, 2017; Revised version received: January 14, 2017; Accepted: January 17, 2017; Published: January 25, 2017.