AbstractChanging wood color with physical treatment is an alternative method to avoid the use of chemicals harmful to the environment. However, despite this advantage, there are few studies on the chemistry and colorimetry of pre-hydrolyzed wood. The objective this research was to evaluate the content of extractives, lignin and holocellulose, lightness (L), red matrix (a*), yellow matrix (b*), saturation (C), and tonality angle (H) on strand board particles of pre-hydrolyzed pine and eucalyptus. Strand board particles measuring 23 x 90 x 0.30 mm in width, length, and thickness, respectively, were pre-hydrolyzed at 130, 150, and 170 °C during 7 or 21 minutes. The temperature had a significant effect on the chemical composition of wood. The pre-hydrolysis temperature of 170 °C resulted in higher chemical modifications. The pre-hydrolysis reduced the equilibrium moisture content of pine and eucalyptus particles, especially at 170 °C. Color modification due to pre-hydrolysis varied according to treatment conditions. Pine particles were more resistant color changing than the eucalyptus particles.