Laminated bamboo with radial (LBR) and tangential (LBT) layered structures was manufactured using heat-treated moso bamboo and phenol-formaldehyde adhesive. The physical, mechanical, and fire-related properties were determined. The results showed that layer structure and heat treatment had an impact on the properties of laminated bamboo. LBR showed good mechanical properties, though heat treatment compromised its integrity somewhat. Heat treatment decreased the lightness and increased the green-red index, blue-yellow index, saturation, and total color difference. The samples exhibited changes in two HRR peaks during the combustion process, corresponding to two main release stages for smoke production. Heat-treatment improved the heat rate release (HRR), total heat release (THR), and the effective heat of combustion (EHC) properties, but also they decreased the smoke performances, resulting in higher total suspended particulate (TSP), specific extinction area (SEA), CO, and CO2 yield. LBR is suggested to be manufactured for possible use as a structural engineering material.