Physical, mechanical, and formaldehyde emission properties were studied for medium density fiberboard (MDF) produced with oak (75%) and pine (25%) fibers that had been mechanically refined in the presence of calcite particles. The calcite slurry was prepared at two levels of solids, 1.5% and 3% (10 and 20 kg·m-³). Chips were cooked for 4 min at 185 °C, under 8 bar vapor pressure in an Andritz defibrillator. 1.8% liquid paraffin, 0.72% ammonium sulphate solution, and 11% urea-formaldehyde were added by percentage based on oven-dried wood fibers in the blowline at the exit of the defibrator. The fibers were dried to 11% moisture content. MDF boards (2100 mm × 2800 mm × 18 mm) were created using a continuous hot-press process. The addition of calcite in the course of MDF production resulted in improved physical properties, such as thickness swelling (ThS 24 hours) and water absorption (WA 24 hours). MDF boards prepared with calcite exhibited higher internal bond (IB), modulus of rupture (MOR), and modulus of elasticity (MOE). Resistance to axial withdrawal of screw also was increased by addition of 3% calcite. In addition, the lowest levels of formaldehyde emission were observed for MDF prepared with calcite at the 3% level.