AbstractThe methane potential and influence of the inoculum to substrate ratio of wastewater originating from the production of microcrystalline cellulose (MCC) were studied. Laboratory experiments were carried out in a continuously stirred batch multi-reactor at mesophilic temperature (37 °C). Inoculum to substrate ratios (ISRs) of 2.0, 1.0, 0.8, and 0.5 based on volatile solids (VS) were evaluated. The results demonstrate the suitability of MCC wastewater at ISRs of 2.0, 1.0, and 0.8 with ultimate methane potentials of 333, 297, and 325 mL CH4 per gram of volatile solids added, respectively, which correspond to anaerobic degradabilities of 91.4, 81.7, and 89.3%, respectively, compared to the theoretical potential. The inoculum to substrate ratio of 2.0 provided a faster methane production rate and a kinetic constant of 0.24 d-1, reaching its ultimate yield at day 8 of incubation. The lowest ISR of 0.5 showed the occurrence of process inhibition due to accumulation of acids. Energy estimation suggests that considering the volume and VS of wastewater produced in a MCC mill, a total energy amount of 44,105 GJ/year can be produced, which can be used to replace 29.4% of the natural gas demand.