AbstractWheat straw pulp was mechanochemically processed in a PFI mill in order to improve the effect of laccase/xylanase system (LXS) treatment before bleaching. The delignification and bleachability of the prepared pulp were investigated. The delignification of the prepared pulp could be enhanced with the mechanochemical processing (refining) and LXS treatment. The delignification was increased by 29.8% with refining 7000 revolutions and 5 IU/g enzyme dosage. The LXS treatment after the mechanochemical process could save 28.6% effective usage of chlorine in the subsequent hypochlorite bleaching process, compared with the traditional bio-bleaching. The crystallinity of cellulose was increased by the co-treatment with mechanochemistry and LXS treatment. This result was further supported by the observations from SEM. This co-treatment with mechanochemistry and bio-treatment enhanced the delignification and bleachability of pulp.