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Lan, T., Lin, T., and Qin, Y. (2020). "Enhancement of enzyme hydrolysis by increasing the zeta potential to reduce non-productive cellulase adsorption on sugarcane bagasse treated with liquid hot water," BioRes. 15(3), 5965-5974.

Abstract

The enhancement of enzymatic hydrolysis is important for the biorefinery industry of lignocellulose. Changing the pH of hydrolysis is a simple and direct way to improve hydrolysis efficiencies. In this study, the enzymatic hydrolysis efficiencies of sugarcane bagasse (SCB) treated with liquid hot water (LHW) were 56.7% and 65.5% at pHs of 4.8 and 5.5, respectively. The result of cellulase adsorption on the LHW treated SCB showed that the non-productive adsorption was smaller at pH 5.5, which might tend to enhance hydrolysis. The surface hydrophobicity of lignin was larger at pH 5.5. This suggested that the hydrophobic interaction was not dominant because a strong hydrophobicity force can cause more non-productive adsorption of cellulase with lignin. At pH 5.5, the surface negative charges of lignin and cellulase increased. Therefore, the electrostatic repulsive force between lignin and cellulase increased, leading to less of the non-productive adsorption of cellulase on lignin. In addition, the cellulase desorption from the LHW treated SCB also increased at pH 5.5. This was beneficial in increasing the possibility of cellulase re-adsorption in new binding sites on cellulose and promoting enzyme hydrolysis efficiency.


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Enhancement of Enzyme Hydrolysis by Increasing the Zeta Potential to Reduce Non-productive Cellulase Adsorption on Sugarcane Bagasse Treated with Liquid Hot Water

Tianqing Lan,a,b Tong Lin,a and Yuyue Qin a,*

The enhancement of enzymatic hydrolysis is important for the biorefinery industry of lignocellulose. Changing the pH of hydrolysis is a simple and direct way to improve hydrolysis efficiencies. In this study, the enzymatic hydrolysis efficiencies of sugarcane bagasse (SCB) treated with liquid hot water (LHW) were 56.7% and 65.5% at pHs of 4.8 and 5.5, respectively. The result of cellulase adsorption on the LHW treated SCB showed that the non-productive adsorption was smaller at pH 5.5, which might tend to enhance hydrolysis. The surface hydrophobicity of lignin was larger at pH 5.5. This suggested that the hydrophobic interaction was not dominant because a strong hydrophobicity force can cause more non-productive adsorption of cellulase with lignin. At pH 5.5, the surface negative charges of lignin and cellulase increased. Therefore, the electrostatic repulsive force between lignin and cellulase increased, leading to less of the non-productive adsorption of cellulase on lignin. In addition, the cellulase desorption from the LHW treated SCB also increased at pH 5.5. This was beneficial in increasing the possibility of cellulase re-adsorption in new binding sites on cellulose and promoting enzyme hydrolysis efficiency.

Keywords: Cellulase adsorption; Enzymatic hydrolysis; Hydrophobicity; Zeta potential; Sugarcane bagasse

Contact information: a: Faculty of Agriculture and Food, Kunming University of Science and Technology, Kunming, Yunnan 650500, China; b: State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, No. 381 Wushan Rd., Guangzhou, 510640, China;

*Corresponding author: rabbqy@163.com

INTRODUCTION

The utilization of renewable biomass energy can alleviate the fossil energy crisis and environmental problems. Therefore, it has received more attention in recent years (Alya and Steven 2012). Lignocellulosic biomass has become an important raw material for renewable energy production due to the abundant reserves and re-application possibilities. However, the enzymatic hydrolysis efficiency of lignocellulose still needs further improvement. Characteristics of cellulase adsorption on lignocellulosic substrates and interactions between substrates and cellulase are the key influencing factors of enzymatic hydrolysis efficiency (Zheng et al. 2016; Zhai et al. 2018).

Cellulase activity is generally considered to be optimal at pH 4.5 to 5.0. However, some studies showed that pretreatment methods can affect the optimal pH of enzymatic hydrolysis of lignocellulose (Lan et al. 2013; Lai et al. 2018). Therefore, elevating the pH of enzymatic hydrolysis is efficient to improve hydrolysis efficiency (Lai et al. 2018). However, the effect mechanism of pH on hydrolysis is still not revealed clearly.

The objective of this study was to explore why the enzymatic hydrolysis efficiency of the LHW treated SCB at pH 5.5 was higher than at pH 4.8. This was accomplished by investigating cellulase adsorption profiles on the LHW treated SCB, the hydrophobicity and zeta potentials of lignin and cellulase, and the pore properties and chemical groups on the substrates’ surface of hydrolysis residues. This study examined the effect of pH on the enzymatic hydrolysis of cellulose, adsorption profiles of lignocellulose and cellulase, and the surface characteristics of lignocellulosic substrates.

EXPERIMENTAL

Materials

Sugarcane bagasse was provided by the Guitang Sugar Refinery (Guigang, China). The size of sugarcane bagasse pieces was about from 0.1 × 0.1 × 0.1 to 0.1 × 1.0 × 2.0 cm3. The cellulase CTec2 (147 FPU per mL, 84.43 mg protein per mL) was purchased from Novozymes (Tianjin, China). All chemicals were analytical grade.

Pretreatment

The SCB pieces were dried to the constant weight at 30 °C in an oven but not ground. The ratio of dried SCB to water was 1 to 20. The LHW pretreatment was conducted at 170 °C for 20 min in the rotating steam-jacketed pressure vessel (ZQS1-15, Machinery Works in Shanxi University of Science and Technology, Shanxi, China).

Enzymatic Hydrolysis

The enzyme hydrolysis of the LHW treated SCB was performed at 50 °C and 180 rpm for 72 h. This was performed in a 50 mM citric acid/citrate buffer (pH 4.8 or 5.5) with a substrate loading of 2% and an enzyme loading of 7.5 FPU per g of dried LHW treated SCB. The reducing sugar content in hydrolysate was determined by the dinitrosalicylic acid (DNS) method (Ghose 1987). The enzyme hydrolysis efficiency was calculated according to Eq. 1,

Hydrolysis efficiency (%) = (RS × 0.9 × 100%) ÷ WC (1)

where RS (mg) is the reducing sugar weight in enzymatic hydrolysate, 0.9 is the conversion coefficient between glucose and glucan, and WC is the carbohydrate weight in the dried LHW treated SCB used for enzymatic hydrolysis.

Enzyme Adsorption and Desorption

Productive and non-productive adsorption

The extraction of cellulose was performed according to the reported method (Jin et al. 2019). The lignin was isolated as described in the literature (Lou et al. 2013). The contents of the extracted cellulose and lignin were 88.55±0.17% and 75.32±0.59%, respectively. The composition was analyzed according to the protocol recommended by the National Renewable Energy Laboratory (Sluiter et al. 2012). The experiments of productive and non-productive adsorption of cellulase on the LHW treated SCB were conducted in 50 mM citric acid/citrate buffer (pH 4.8 or 5.5) at 4 °C using the extracted cellulose or isolated lignin as the substrate. The substrate loading was 1% (weight per volume). The substrate was kept in the buffer for 2 h, and then cellulase was added. The enzyme loading was 160 mg of protein per g of dry substrate. The mixture was incubated at 4 °C and 180 rpm for 2 h and then centrifuged at 8000 rpm for 15 min. The protein content in the supernatant was determined by the Bradford method (Liu et al. 2017). The adsorbed protein was calculated by subtracting the free protein in the supernatant from the total used cellulase protein.

Desorption

The solid obtained after centrifugation in the adsorption experiment was put in 10 mL of 50 mM citric acid/citrate buffer solution, and then incubated at 4 °C and 180 rpm for 2 h. Next, it was centrifuged at 8000 rpm for 15 min. The free protein in the supernatant was the desorbed cellulase (Lou et al. 2013).

Adsorption kinetics

The experiment was carried out as described in the Productive and Non-Productive Adsorption section. The only difference was that before centrifugation, the incubation time was 5, 10, 20, 30, 60, 90, and 120 min, respectively. The fitting of the adsorption kinetics was based on the pseudo-first-order adsorption kinetic model (Guo and Wang 2019). This model was calculated according to Eq. 2,

(dqt) ÷ dt = k1 (qe  qt) (2)

where k(min-1) is the first-order rate constant, qe (mg protein per g of dry SCB) and q(mg protein per g of dry SCB) are the cellulase adsorption amounts at the adsorption equilibrium, and t (min) is the adsorption time, respectively.

Hydrophobicity

The experiment was performed according to the published literature (Huang et al. 2017). The isolated lignin of 1, 2, 3, 4, and 5 g per L was individually put in the 50 mM citric acid/citrate buffer solution (pH 4.8 or 5.5) containing rose bengal with 40 mg per L. The mixture was incubated at 50 °C and 180 rpm for 2 h. It was then separated by centrifugation at 8000 rpm for 15 min. The supernatant was determined at 543 nm using a UV-Visible spectrophotometer (UV-1800PC, Mapda Instrument Limited Company, Shanghai, China). The ratio of adsorbed rose bengal to free rose bengal in the supernatant was the ordinate, and the lignin content was the abscissa. The slope represented the hydrophobicity of lignin.

Zeta Potential

Cellulase and lignin of 0.1% (weight per volume) were prepared with citric acid/citrate buffer solutions at pH 4.8 or 5.5. Each experiment was done in triplicate using a zeta potentiometer (Zetasizer Nano ZS90, Malvern, Malvern, England) (Yang et al. 2017).

Brunauer-Emmett-Teller (BET) and Fourier Transform Infrared (FTIR)

The specific surface area, pore volume, and average pore diameter of hydrolysis residues were tested by a BET analyzer (ASAP 2460, Micromeritics, Georgia, USA). The chemical groups were determined by a FTIR spectrometer (TENSOR27, Bruker, Karlsruhe, Baden-Wurttemberg, Germany).

Statistical Analysis

Statistical analysis was conducted by a student’s t test. Origin 8.5 (Origin8.5, OriginLab, Northampton, Massachusetts, USA) was used for the data analysis. A p-value less than 0.05 indicated a significant difference.

RESULTS AND DISCUSSION

Enzymatic Hydrolysis

The optimal pH value of enzymatic hydrolysis of pure cellulose was pH 4.8, which has been widely accepted and used for the saccharification of lignocellulose. However, Lou et al. (2013) suggested that different conditions should be considered, since lignocellulose is different from pure cellulose without lignin. It was found that the enzymatic hydrolysis of lignocellulose at a pH value of higher than 5.5 was higher than that at pH 4.8. In Fig. 1, the enzymatic hydrolysis efficiencies of the LHW treated SCB at pH 4.8 and 5.5 are listed. The results showed that the enzymatic hydrolysis efficiency of the LHW treated SCB at pH 5.5 (65.46%) was higher (P < 0.05) than at pH 4.8 (56.70%). It has been reported that the enzymatic hydrolysis efficiency of pretreated lignocellulose was closely related to the lignocellulosic surface characteristics. This includes the adsorption and desorption profiles of cellulase with lignocellulose and the zeta potential and hydrophobicity of the lignocellulosic substrate (He et al. 2017; Lu et al. 2017a; Lai et al. 2018). Therefore, to discover the reason why the hydrolysis of the LHW treated SCB at pH 5.5 was higher than at pH 4.8, the surface characteristics of the LHW treated SCB were determined.

Fig. 1. Enzymatic hydrolysis efficiencies of the LHW treated SCB at pHs of 4.8 and 5.5

Fig. 2. Adsorption kinetics of cellulase with the LHW treated SCB at pHs of 4.8 and 5.5

Cellulase Adsorption and Desorption

In Fig. 2, the adsorption kinetics of the LHW treated SCB at pHs of 4.8 and 5.5 are demonstrated. In Table 1, the fitting parameters of pseudo-first-order cellulase adsorption kinetics of the LHW treated SCB are given. From Table 1, it was found that the cellulase adsorption profiles on the LHW treated SCB at two pHs were well fitted with the pseudo-first-order adsorption kinetics (R2 approximated to 1). The values of k1 at pH 4.8 and pH 5.5 indicated that the cellulase adsorption at a 5.5 pH was quicker than at a 4.8 pH, which might promote the enzymatic hydrolysis of the LHW treated SCB.

Additionally, the results from Fig. 2 showed that the maximum adsorption amounts of the LHW treated SCB at pHs of 4.8 and 5.5 were 40.6 and 38.4 mg protein per g of substrate at 30 and 20 min, respectively. This was extremely similar to the fitting values of 40.4 and 37.9 mg protein per g of substrate in Table 1. The hydrolysis efficiency at pH 5.5 was higher, but the adsorption amount of cellulase was lower. Therefore, it was assumed that the non-productive adsorption of cellulase at pH 5.5 would be lower.

Table 1. Fitting Parameters of Pseudo-First-Order Adsorption Kinetics of LHW Treated SCB

The productive and non-productive adsorptions were subsequently determined in this study, representing the adsorptions of cellulase on cellulose and lignin extracted from the LHW treated SCB, respectively. In Fig. 3, the productive and non-productive adsorptions are presented. The results showed that the productive adsorption at these two pHs had no significant difference (p > 0.05), and the non-productive adsorption at pH 5.5 was smaller. In Fig. 3, the desorption amounts at the two pH levels are also shown. The desorption amount at the 5.5 pH level was larger. A higher productive adsorption amount and a lower non-productive adsorption amount of cellulase were helpful for the enzymatic hydrolysis efficiency of cellulose (Zheng et al. 2020). In addition, a larger desorption amount of cellulase was also beneficial to the hydrolysis of cellulose. This is because it was more possible for the desorbing cellulase to adsorb again on the new binding sites of cellulose with cellulase (Hao et al. 2019).

Fig. 3. Productive adsorption, non-productive adsorption, and desorption of cellulase

Non-productive adsorption of cellulase on lignin was mainly due to hydrophobic and electrostatic interactions (Huang et al. 2017). Thus, hydrophobicity and zeta potentials of the LHW treated SCB at the two pH levels of 4.8 and 5.5 should be further studied.

Hydrophobicity

The data on the hydrophobicity of lignin and cellulase are listed in Table 2. The hydrophobicity of lignin at the 5.5 pH level was 0.38 L per g, which was higher than 0.09 L per g at the 4.8 pH level. Additionally, the hydrophobicity of cellulase (3.64 L per g) at the 5.5 pH level was also higher than 2.02 L per g at the 4.8 pH level. A higher hydrophobicity of lignocellulose can produce a stronger hydrophobic interaction force between the substrate and cellulase (Lai et al. 2018). Therefore, the hydrophobic interaction between lignin and cellulase at the 5.5 pH level was higher than at the 4.8 pH level. However, the non-productive adsorption amount of cellulase on lignin at the 5.5 pH level was lower than that at the 4.8 pH level in this study. Therefore, the hydrophobic interaction did not play a dominant role in the non-productive adsorption of cellulase on lignin. The similar phenomenon was observed in other reported literatures (Lu et al. 2017b; Zhang et al. 2016). Therefore, the electrostatic interaction force between lignin and cellulase might be dominant. To verify the effect of electrostatic interaction on non-productive adsorption, zeta potentials of cellulase and lignin were measured, respectively.

Table 2. Hydrophobicity and Zeta Potential of Cellulase and Lignin

Zeta Potential

The zeta potential values of cellulase and lignin are shown in Table 2. The zeta potentials of lignin and cellulase at two pHs were all negative. Therefore, there were electrostatic repulsion forces between lignin and cellulase. At the 5.5 pH level, the absolute values of the zeta potential of lignin and cellulase both increased, indicating that the electrostatic repulsion force between cellulase and lignin increased. The increase in the repulsion force caused the decrease in the non-productive adsorption amount of cellulase on lignin. Therefore, in this study, the electrostatic interaction between lignin and cellulase might mainly be responsible for the decrease in the non-productive adsorption amount of cellulase on lignin at the 5.5 pH level. As a result, the enzymatic hydrolysis efficiency at the 5.5 pH level was improved.

BET Analysis

In order to explore the hydrolysis mechanism of lignocellulose, except for the adsorption profiles of cellulase, the hydrolysis residues of the LHW treated SCB should also be studied due to their importance (Pihlajaniemi et al. 2016). In Table 3, the specific surface area, pore volume, and average pore diameter of hydrolysis residues of the LHW treated SCB are shown. The specific surface area, pore volume, and average pore diameter of the LHW treated SCB at the 5.5 pH level were all higher than those at the 4.8 pH level, suggesting that the enzyme hydrolysis was more effective at a 5.5 pH.

Table 3. Specific Surface Area, Total Pore Volume, and Average Pore Diameter of the Enzymatic Hydrolysis Residues of the LHW-treated SCB

FTIR Analysis

In Fig. 4, the FTIR spectra of enzymatic hydrolysis residues of the LHW treated SCB are shown. The peaks at 2930, 1730, 1604, and 1083 cm-1 respectively represented the methoxy groups, the ester groups, the aromatic ring structure and the aromatic methyl ether bridges of lignin (Kang et al. 2012; Guo et al. 2014; Zehra et al. 2019). These groups are all hydrophobic (Li et al. 2018; Lavagna et al. 2019; Yu et al. 2019; Chai et al. 2020). The transmittance intensities at these peaks of the hydrolysis residue at the 5.5 pH level were smaller, suggesting that the contents of the hydrophobic groups of hydrolysis residue at pH 5.5 were higher than that at pH 4.8. The hydrophobic interaction was stronger between lignin and cellulase at the 5.5 pH level. This was consistent with the hydrophobicity results.

Fig. 4. FTIR spectra of enzymatic hydrolysis residues of the LHW treated SCB at pH (A) 4.8 and (B) 5.5

CONCLUSIONS

  1. The enzymatic hydrolysis efficiencies of the LHW treated SCB were 56.7% and 65.5% at pH 4.8 and 5.5 respectively, with a significant difference (P < 0.05). This was due to the decrease in the non-productive adsorption of cellulose on the LHW treated SCB at the 5.5 pH level.
  2. The increase in the hydrophobicity of lignin and cellulase at the 5.5 pH level was not the dominant reason for the improvement in the enzymatic hydrolysis. The increasing zeta potentials of lignin and cellulase was mainly responsible for the decrease in the non-productive adsorption of cellulase at the 5.5 pH level.

ACKNOWLEDGMENTS

This work was supported by the National Natural Science Foundation of China (Grant No. 21766014) and the State Key Laboratory of Pulp and Paper Engineering (Grant No. 201811).

REFERENCES CITED

Alya, L., and Steven, C. (2012). “Lignocellulosic biomass for bioethanol production: current perspectives, potential issues and future prospects,” Progress in Energy & Combustion Science 38(4), 449-467. DOI: 10.1016/j.pecs.2012.03.002

Chai, X., Song, X., He, H., Fan, H., and Liang, D. (2020). “Effects of adsorbate molecular space conformation on the adsorption capacity of porous carbon materials: A case study of propylene glycol methyl ether,” Science of The Total Environment 712, 135495. DOI: 10.1016/j.scitotenv.2019.135495

Ghose, T. (1987). “Measurement of cellulase activities,” Pure & Applied Chemistry 59(2), 257-268. DOI: 10.1351/pac198759020257

Guo, F., Shi, W., Sun, W., Li, X. Z., Wang, F., F., Zhao, J., and Qu, Y. (2014). “Differences in the adsorption of enzymes onto lignins from diverse types of lignocellulosic biomass and the underlying mechanism,” Biotechnology for Biofuels 7, 38. DOI: 10.1186/1754-6834-7-38

Guo, X., and Wang, J. (2019). “A general kinetic model for adsorption: Theoretical analysis and modeling,” Journal of Molecular Liquids 288, 111100. DOI: 10.1016/j.molliq.2019.111100

Hao, X., Li, Y., Wang, J., Qin, Y., and Zhang, J. (2019). “Adsorption and desorption of cellulases on/from lignin-rich residues from corn stover,” Industrial Crops and Products 139, 111559. DOI: 10.1016/j.indcrop.2019.111559

He, J., Huang, C., Lai, C., Huang, C., and Yong, Q. (2017). “Relations between moso bamboo surface properties pretreated by kraft cooking and dilute acid with enzymatic digestibility,” Applied Biochemistry and Biotechnology 183(4), 1526-1538. DOI:
10.1007/s12010-017-2520-6

Huang, Y., Sun, S., Huang, C., Yong, Q., Elder, T., and Tu, M. (2017). “Stimulation and inhibition of enzymatic hydrolysis by organosolv lignins as determined by zeta potential and hydrophobicity,” Biotechnology for Biofuels 10(1), 162. DOI: 10.1186/s13068-017-0853-6

Jin, K., Liu, X., and Ma, J. (2019). “Delignification kinetics and selectivity in poplar cell wall with acidified sodium chlorite,” Industrial Crops and Products 136, 87-92. DOI: 10.1016/j.indcrop.2019.04.067

Lai, C., Tu, M., Yong, Q., and Yu, S. (2018). “Synergistic effects of pH and organosolv lignin addition on the enzymatic hydrolysis of organosolv-pretreated loblolly pine,” RSC Advances 8(25), 13835-13841. DOI: 10.1039/C8RA00902C

Lan, T., Lou H., and Zhu J. (2013). “Enzymatic saccharication of lignocelluloses should be conducted at elevated pH 5.2~6.2,” BioEnergy Research 6, 476-485. DOI: 10.1007/s12155-012-9273-4

Lavagna, L., Nistico, R., Musso, S., and Pavese, M. (2019). “Hydrophobic cellulose ester as a sustainable material for simple and efficient water purification processes from fatty oils contamination,” Wood Science and Technology 53(1), 249-261. DOI: 10.1007/s00226-018-1060-8

Li, X., Li, M., Pu, Y., Arthur, J., Klettd, A., Thiesd, M., and Zheng, Y. (2018). “Inhibitory effects of lignin on enzymatic hydrolysis: The role of lignin chemistry and molecular weight,” Renewable Energy 123, 664-674. DOI: 10.1016/j.renene.2018.02.079

Liu, Z., Lan, T., Li, H., Gao, X., and Zhang, H. (2017). “Effect of bisulfite treatment on composition, structure, enzymatic hydrolysis and cellulose adsorption profiles of sugarcane bagasse,” Bioresource Technology 223, 27-33. DOI: 10.1016/j.biortech.2016.10.029

Lou, H., Zhu, J., Lan, T., Lai, H., and Qiu, X. (2013). “pH-induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses,” ChemSusChem 6(5), 919-927. DOI: 10.1002/cssc.201200859

Lu, X., Wang, C., Li, X., and Zhao, J. (2017a). “Temperature and pH influence adsorption of cellobiohydrolase onto lignin by changing the protein properties,” Bioresource Technology 245, 819-825. DOI: 10.1016/j.biortech.2017.08.139

Lu, X., Wang, C., Li, X., Zhao, J., and Zhao, X. (2017b). “Studying nonproductive adsorption ability and binding approach of cellobiohydrolase to lignin during bioconversion of lignocellulose,” Energy Fuels 31(12), 14393-14400. DOI: 10.1021/acs.energyfuels.7b02427

Pihlajaniemi, V., Sipponen, M., Kallioinen, A., Nyyssölä, A., and Laakso, S. (2016). “Rate-constraining changes in surface properties, porosity and hydrolysis kinetics of lignocellulose in the course of enzymatic saccharification,” Biotechnology for Biofuels 9(1), 18. DOI: 10.1186/s13068-016-0431-3

Sluiter, A., Hames, B., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., and Crocker, D. (2012). Determination of Structural Carbohydrates and Lignin in Biomass (NREL/TP-510-42618), National Renewable Energy Laboratory, Golden, CO, USA.

Yang, M., Wang, J., Hou, X., Wu, J., Fan, X., Jiang, F., Tao, P., Wang, F., Peng, P., Yang, F., and Zhang, J. (2017). “Exploring surface characterization and electrostatic property of hybrid pennisetum during alkaline sulfite pretreatment for enhanced enzymatic hydrolysability,” Bioresource Technology 24, 1166-1172. DOI: 10.1016/j.biortech.2017.08.046

Yu, H., Hou, J., Yu, S., Liu, S., Wu, Q., Li, L., Liu, Y., and Jiang, J. (2019). “Comprehensive understanding of the non-productive adsorption of cellulolytic enzymes onto lignins isolated from furfural residues,” Cellulose 26, 3111-3125. DOI: 10.1007/s10570-019-02323-1

Zehra, N., Ali, T. M., and Hasnain, A. (2019). “Comparative study on citric acid modified instant starches (alcoholic alkaline treated) isolated from white sorghum and corn grains,” International Journal of Biological Macromolecules 150, 1331-1341. DOI: 10.1016/j.ijbiomac.2019.10.143

Zhai, R., Hu, J., and Saddler, J. (2018). “The inhibition of hemicellulosic sugars on cellulose hydrolysis are highly dependent on the cellulase productive binding, processivity, and substrate surface charges,” Bioresource Technology 258, 79-87. DOI: 10.1016/j.biortech.2017.12.006

Zhang, C., Gleisner, R., Houtman, C., Pan, X., and Zhu, J. (2016). “Sulfite pretreatment to overcome the recalcitrance of lignocelluloses for bioconversion of woody bio-mass,” Biomass Fractionation Technologies for a Lignocellulosic Feedstock Based Biorefinery (Chapter 22) 499-541. DOI: 10.1016/B978-0-12-802323-5.00022-0

Zheng, W., Lan, T., Li, H., Yue, G., and Zhou, H. (2020). “Exploring why sodium lignosulfonate influenced enzymatic hydrolysis efficiency of cellulose from the perspective of substrate-enzyme adsorption,” Biotechnology for Biofuels 13(1), 19. DOI: 10.1186/s13068-020-1659-5

Zheng, Y., Zhang, R., and Pan, Z. (2016). “Investigation of adsorption kinetics and isotherm of cellulase and β-glucosidase on lignocellulosic substrates,” Biomass and Bioenergy 91, 1-9. DOI: 10.1016/j.biombioe.2016.04.014

Article submitted: March 12, 2020; Peer review completed: May 3, 2020; Revised version received and accepted: May 30, 2020; Published: June 17, 2020.

DOI: 10.15376/biores.15.3.5965-5974