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Chiranjeevi, P. V., Pandian, M. R., and Thadikamala, S. (2014). "Enhancement of laccase production from Pleurotus ostreatus PVCRSP-7 by altering the nutritional conditions using response surface methodology," BioRes. 9(3), 4212-4225.

Abstract

Submerged culture conditions for laccase production by Pleurotus ostreatus were optimized by response surface methodology (RSM). A total of six factors, carbon (glucose), nitrogen sources (urea and peptone), 2,5-xylidine (inducer), wheat bran (lignocellulosic material), and medium pH, were optimized. A total of 50 experiments were conducted, and the obtained data were modeled using a second-order polynomial. The optimized conditions show significant improvement in laccase expression, by approximately 3.5-fold (12,124 U/L).
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