NC State
Kou, X., Yang, R., Zhao, J., Lu, J., and Liu, Y. (2013). "Enzymatic saccharification and L-lactic acid fermentation of corn stover pretreated with liquid hot water by Rhizopus oryzae," BioRes. 8(4), 4899-4911.


Corn stover was pretreated with liquid hot water (LHW) to enhance its enzymatic hydrolysis and L-lactic fermentation. The cellulose conversion rate and L-lactic acid concentration were used to evaluate LHW pretreatment performance. Results showed that the optimum conditions for the LHW pretreatment of corn stover are a reaction temperature of 190 °C for 20 min and a solid-to-liquid ratio of 1:10. The cellulase loading was 30 filter paper units per gram of oven-dried, water-insoluble solid. These conditions resulted in 92.3% conversion of cellulose to glucose. Sequential hydrolysis and fermentation using pretreated water-insoluble solid (WIS) produced an L-lactic acid concentration of 45 g/L. This study indicated that LHW pretreatment of corn stover is a suitable method for achieving high cellulose conversion and L-lactic acid concentration.
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