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Martín-Sampedro, R., Rodríguez, A., Requejo, A., and Eugenio, M. E. (2012). "Improvement of TCF bleaching of olive tree pruning residue pulp by addition of a laccase and/or xylanase pre-treatment," BioRes. 7(2), 1488-1503.


This study aimed at assessing the biobleachability of soda pulps obtained from olive tree pruning residue. The enzymatic (LMS) pre-treatment was applied prior to a simple totally chlorine free (TCF) bleaching sequence, consisting of an alkaline extraction and a hydrogen peroxide stage. Additionally, the effect of adding xylanase jointly with or prior to LMS was evaluated. All of these enzymatic pre-treatments were associated with an enhancement of the bleaching sequence. The best results were found when both enzymes were applied in the same stage: lowest hydrogen peroxide consumption (63 percent); kappa number, 11.6; brightness, 46 percent ISO. The mechanical properties observed were similar to those reported by other authors who have studied pulps from olive tree pruning residue. Finally, bleached pulps were subjected to accelerated ageing in order to assess the evolution of brightness and colorimetric properties. Although biobleached pulps showed lower stability upon ageing, the best optical properties, even after ageing, were observed in pulps treated with both xylanase and laccase.
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