AbstractLaccases (benzenediol:oxygen oxidoreductase, EC 18.104.22.168) are multi-copper oxidases that are widely distributed among plants, insects, and fungi. They have been described in different genera of ascomycetes, some deuteromycetes, and mainly in basidiomycetes. These enzymes catalyze the one-electron oxidation of a wide variety of organic and inorganic substrates, including mono-, di-, and polyphenols, aminophenols, methoxyphenols, aromatic amines, and ascorbate, with the concomitant four electron reduction of oxygen to water. Laccase is currently the focus of much attention because of its diverse applications, such as delignification of lignocellulosics, crosslinking of polysaccha-rides, bioremediation applications, such as waste detoxification, and textile dye transformation, food technologic uses, personal and medical care applications, and biosensor and analytical applications. This review helps to understand the properties of this important enzyme for efficient utilization for its biotechnological and environmental applications.