AbstractThis study investigated the effect of ammoniation pretreatment of corn stalk (CS) with different temperatures, ammonia proportions, and processing times on lignocellulolytic enzyme production in solid-state fermentation (SSF) by Lentinus edodes L-8. The total N content and lignocellulose contents of ammoniated corn stalk were determined for analysis of the effect of ammoniation pretreatment on lignocellulose structure. The variation patterns of enzyme activity were analyzed according to the enzyme data determined every 2 days during the fermentation. A 4% w/w high-temperature ammoniation pretreatment had a significant effect on cellulase production, and the highest enzyme activity reached almost triple that of the control group. The results also showed that ammoniation pretreatment inhibited the generation of ligninases, such that ligninases appeared later and at lower activities in experimental groups compared to the control group.