Ajith, Tripathi, K., Yadav, D. K., Kaushik, P., Rana, V. S., Shakil, N. A., Pankaj, Koli, P., and Ren, Y. (2024). "Nematicidal efficacy of Cymbopogon nardus, Pelargonium graveolens essential oils and Annona squamosa extract against Meloidogyne incognita and M. graminicola," BioResources 19(4), 9685–9707.
Abstract
A crude hexane extract of seed kernel was processed into fatty acid methyl esters (FAME) using methanolic-sulfuric acid. Herbal mixtures (seed extracts of A. squamosa and essential oils of Cymbopogon nardus (citronella) and Pelargonium graveolens (geranium)) were evaluated against root knot nematode (Meloidogyne spp). The mortality of J2s of M. incognita in hexane and methanol extracts (AHE+AME) of A. squamosa with geranium oil (GO) ranged from 50.75-89.75% to 71.50-99.00% at 31.25 to 1000 μgmL-1 in 24 to 96 hours. The number of galls after CO+AHE+AME treatment was 4.0 ± 0.0 and 5.67 ± 0.58 galls/seedling and were recorded at 1000 and 500 μgmL-1 treatments on 30 days after inoculation, compared to carbofuran (3.0 ± 0.0 and 4.3 ± 0.6 galls/seedling) at 1000 and 500 μgmL-1 and velum prime (3.6 ± 0.6 and 4.6 ± 0.6 galls/seedling) at 1000 and 500 μgmL-1. The maximum shoot length of brinjal seedlings was recorded in CO+AHE+AME treatment compared to carbofuran (47.3 ± 0.6cm) and velum prime (48.7 ± 0.6 cm) at 1000 μg mL-1 in soil drenching application. Methanol and hexane extracts (AHE+AME) obtained from Annona squamosa seeds were investigated for their nematicidal properties. M. graminicola in direct-seeded rice at the nursery level and M. incognita in brinjal (eggplant) were controlled for up to sixty days when treated with these mixtures.
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Nematicidal Efficacy of Cymbopogon nardus, Pelargonium graveolens Essential Oils and Annona squamosa Extract against Meloidogyne incognita and M. graminicola
Ajith M.,a Kailashpati Tripathi,b Dinesh Kumar Yadav,c Parshant Kaushik,a Virendra Singh Rana,a Najam Akhtar Shakil,a Pankaj,d Pushpendra Koli,e,f and Yonglin Ren f
A crude hexane extract of seed kernel was processed into fatty acid methyl esters (FAME) using methanolic-sulfuric acid. Herbal mixtures (seed extracts of A. squamosa and essential oils of Cymbopogon nardus (citronella) and Pelargonium graveolens (geranium)) were evaluated against root knot nematode (Meloidogyne spp). The mortality of J2s of M. incognita in hexane and methanol extracts (AHE+AME) of A. squamosa with geranium oil (GO) ranged from 50.75-89.75% to 71.50-99.00% at 31.25 to 1000 μgmL-1 in 24 to 96 hours. The number of galls after CO+AHE+AME treatment was 4.0 ± 0.0 and 5.67 ± 0.58 galls/seedling and were recorded at 1000 and 500 μgmL-1 treatments on 30 days after inoculation, compared to carbofuran (3.0 ± 0.0 and 4.3 ± 0.6 galls/seedling) at 1000 and 500 μgmL-1 and velum prime (3.6 ± 0.6 and 4.6 ± 0.6 galls/seedling) at 1000 and 500 μgmL-1. The maximum shoot length of brinjal seedlings was recorded in CO+AHE+AME treatment compared to carbofuran (47.3 ± 0.6cm) and velum prime (48.7 ± 0.6 cm) at 1000 μg mL-1 in soil drenching application. Methanol and hexane extracts (AHE+AME) obtained from Annona squamosa seeds were investigated for their nematicidal properties. M. graminicola in direct-seeded rice at the nursery level and M. incognita in brinjal (eggplant) were controlled for up to sixty days when treated with these mixtures.
DOI: 10.15376/biores.19.4.9685-9707
Keywords: Antinemic activity; Annona squamosa; Citronella oil; Geranium oil; Herbal extract
Contact information: a: Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India; b: ICAR-National Research Centre on Seed Spices, Ajmer, Rajasthan 305206, India; c: ICAR-Indian Institute of Soil Science, Bhopal, MP 462038, India; d: Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi 110012, India; e: ICAR-Indian Grassland and Fodder Research Institute, Jhansi, UP 284003, India; f: College of Environmental and Life Sciences, Murdoch University, Murdoch, WA 6150, Australia; *Corresponding author: ranavs2000@yahoo.com; kolipushpendra@gmail.com; y.ren@murdoch.edu.au
INTRODUCTION
Plant-parasitic nematodes (PPNs) responsible for major damage to important crops worldwide. A recent report showed that PPNs lead to an average yield reduction of 13.5% in 40 key food and export crops, which corresponds to USD 358.24 billion annually (Abd-Elgawad et al. 2024). Nematodes are known to infest the roots of vegetables, cereals, and pulses, causing a substantial loss in yield. Rice is a staple food for about four billion people. It is primarily grown in Asia. India, the second-largest rice producer, is facing an emerging problem due to nematode infestation in rice cultivation (Pathak et al. 2022). Approximately 4,100 species of plant-parasitic nematodes are described, causing up to US$80 billion in crop losses annually (Decraemer and Geraert 2006). Species of Meloidogyne can parasitize almost all vascular plants, leading to complete crop loss (El-Nagdi et al. 2014). Crop losses are often higher in developing countries due to a lack of awareness about nematode infestations (Moens et al. 2009; Jain et al. 2012; Yadav et al. 2021a).
Meloidogyne graminicola, a phytopathogenic nematode, significantly impacts rice cultivation, causing over 20% of total economic loss (Patil et al. 2020). These nematodes form hook-shaped galls at rice root tips. M. incognita, found in tropical and subtropical regions, cause substantial damage to vegetable crops, including brinjal, causing significant yield losses due to disrupted nutrient uptake and plant growth (Jones et al. 2013; Ghosh 2021; Yadav et al. 2022).
Synthetic nematicides are highly effective against nematodes. However, their use has declined in recent decades due to their toxicity to non-target organisms and the environment (Yadav et al. 2021b). Integrated pest management (IPM) strategies offer promising alternatives to mitigate nematode populations in soil (Ntalli et al. 2010; Ajith et al. 2020). This study highlights various IPM techniques, including crop rotation, mulching with Tagetes and Crotalaria species, soil amendment, and the utilization of biocontrol agents such as Trichoderma spp., Paecilomyces lilacinus, and Pseudomonas fluorescens. Additionally, non-host crops and nematode-resistant varieties are discussed as environmentally benign management strategies.
Botanical pesticides (such as neem oil, pyrethrins, clove essential oil, and karanj oil) offer an alternative to synthetic ones, being less harmful to the environment and human health (Akhtar et al. 2000; Acheuk et al. 2022). Their biodegradability and compatibility with biocontrol agents make them useful (Kumar et al. 2022). Plant-derived secondary metabolites (estragole, γ-eudesmol, trans-anethole/geraniol) are gaining attention for effectively targeting root-knot nematodes while sparing non-target organisms (Jirovetz et al. 2006).
The use of seed extract and essential oils from plant origins in the management of nematodes in agricultural crops has not been much explored at pot levels except under in vitro conditions (Jirovetz et al. 2006). That is the reason why up to the present, no nematicidal products of plant origin, either in the form of seed extracts, essential oils, or herbal mixture are available to end users (Ntalli et al. 2011a,b). Essential oils, a complex of volatile chemical compounds with multiple modes of action, are reported to possess antifungal, antibacterial, and insecticidal activities (Mossa et al. 2016). Citronella essential oil and its isolated compounds were screened in vitro for antifungal potential against F. verticillioides and D. maydis using poisoned food technique. Citronella essential oil (ED50 0.095 mg/ml) was most effective against F. verticillioides (Kaur et al. 2021). Botanicals were toxic to S. frugiperda larvae at a single concentration (1%), and the maximum mortality was observed after 72 h. Geranium oil resulted in the highest mean larval mortality after 24 h of treatment (40%) (Keerthi et al. 2023).
However, recent studies highlight the potential efficacy of essential oils in managing nematodes in rice, brinjal, in field and greenhouse environments (Choi et al. 2007; Andres et al. 2012; Faria et al. 2013; Julio et al. 2017; Rajasekharan et al. 2020). A gap in research could involve doubts about how well mixing seed extracts (Annona squamosa) and essential oils from certain plants Cymbopogon nardus and Pelargonium graveolens can control root-knot nematodes (M. incognita and M. graminicola). While separate studies have shown good results, nobody has studied how effective they are when used together (Jardim et al. 2018).
In vitro studies showed that mixture of essential oil (Cymbopogon nardus, and Pelargonium graveolens) and seed extracts (Annona squamosal) could be an important source of nematicidal agents, especially for the management of M. graminicola and M. incognita in rice and brinjal.
There are very few studies on the nematicidal activity of essential oil (Shakil et al. 2004) against Meloidogyne incognita but in the present work first time we have evaluated the mixture of seed extracts from Annona squamosa and essential oils from Cymbopogon nardus and Pelargonium graveolens against M. incognita. Exploring the untapped potential of these botanical products as nematicides could pave the way for new alternatives.
In this study, the essential oils from Pelargonium graveolens (geranium) and Cymbopogon nardus (citronella) and seed extract from the Annona squamosa were isolated, and their chemical profiling was done by gas chromatography with flame-ionization detection (GC-FID) and gas chromatography–mass spectrometry (GC-MS). The mixtures of essential oils (P. graveolens and C. nardus) and seed extract (A. squamosa) were evaluated for nematicidal activity against M. graminicola and M. incognita both under in vitro and pot conditions.
EXPERIMENTAL
Preparation of Hexane Extract and Essential Oils Extraction
Fresh seeds of Annona squamosa were procured from the Khari Bably market in New Delhi in September 2021. The seeds were ground into a fine powder using a grinder. Crude fatty oil was then extracted from the powdered seeds using hexane as a solvent in a Soxhlet apparatus (Borosil®, India) over a period of 48 hours. The resulting extract was concentrated using a rotary evaporator.
The essential oils were isolated from shed dried C. nardus (citronella) leaves (400 g) and P. graveolens (geranium) aerial parts (500 g, leaves and inflorescence) by hydrodistillation method using Clevenger-type apparatus for 4 h and repeated thrice. The distillate was extracted with diethyl ether and the ethereal layer was dried over anhydrous sodium sulphate. The ether was distilled off on a gently heated water bath and stored in amber vials in a refrigerator until analysis and stored at 4 °C. The yields of the oils in C. nardus and P. graveolens were found to be 0.97% and 0.84%, respectively.
Preparation of Fatty Acid Methyl Esters
The crude hexane extract containing mainly total oil was converted into fatty acid methyl ester using an acidic esterification method (Saxena et al. 2005). Briefly, 200 mg of crude total oil was dissolved in 20 mL of sulfuric acid-methanol (1.0%) (0.20 mL H2SO4 in 19.80 mL methanol) and was refluxed in a boiling water bath for 2 h. The reaction mixture was cooled and dried under reduced pressure (50 mbar and 45 °C). The dried mixture was dissolved in 20 mL of distilled water and neutralized with a saturated solution of sodium carbonate followed by extraction of methyl esters with diethyl ether (Rana et al. 2008). The ethereal mixture of methyl esters was dried over anhydrous sodium sulphate. The ether was removed from the mixture and stored at 4 °C.
GC-FID and GC/MS Analysis
The fatty acid methyl esters were analyzed by GC-FID (Shimadzu GC 2010 Plus) fitted with a capillary column, SH Rtx-5 (30 m × 0.25 mm × 0.25 µm film thickness). GC conditions were: N2 as carrier gas (1.0 mL/min); injector and flame ionization detector temperatures were 250 ℃ and 260 ℃, injected volume (0.3 µL, 1000 μg mL-1 in hexane); and injection mode (split ratio 1:20). The column temperature was held at 60 ℃, then increased to 250 ℃ at 3 ℃/min. The relative percentage composition of the oils were determined by the normalization method from the GC peak areas and then calculated as the mean value of two injections, without using correction factors.
A GC/MS (Focus-Polaris Q; Thermo, USA) bench top quadrupole ion trap mass spectrometer outfitted with a ZB-5 capillary column (30 m × 0.25 mm internal diameter (id) film thickness 0.25 mm) was used to analyze the fatty acid methyl esters. The oven was preheated to 60 °C and programmed to rise to 250 °C in 3 min. The injection volume was 0.2 µL (1000 μg mL-1 in hexane), the split flow was 20 mL min-1, and helium was employed as the carrier gas at a flow rate of 1 mL min-1 (split mode, 1:20). The temperatures of the injector and transfer line were 220 and 240 ºC, respectively. The mass spectrometer’s source temperature was 220 ºC. Analysis was done using the mass range of 40 to 400 atomic mass unit (a.m.u.) at 70 eV in electron ionization mode. Identification of individual compounds was carried out by comparing the retention time and mass spectra with certified reference materials (Sigma-Aldrich), as well as mass spectra in the NIST Mass Spectral Library Version 2.0 d (2005).
Nematicidal Activity
Preparation of test samples
The test solution (1.0%) of mixture of A. squamosa hexane extract (AHE) and A. squamosa methanol extract from A. squamosa seeds, when mixed in equal proportion with citronella oil or geranium oil, was prepared in distilled water with polysorbate 80 (Tween-80) (4%) followed by mixing the ingredients using a lab stirrer for 1 h (Ibrahim et al. 2006). Different concentrations (1000, 500, 250, 125, 62.5, and 31.25 μg mL-1) of the test solutions were prepared by serial dilution for their evaluation against M. graminicola and M. incognita. The stock solution (1.0%) of carbofuran and velum prime prepared in water and applied via root dipping and soil drenching using two commercial nematicides, carbofuran 3G (Furadon by Crystal crop) and velum prime (Fluopyram 34.48% SC by Bayer) and desired concentration (62.5 and 31.25 μg mL-1) were prepared by serial dilution from stock the solution.
Isolation of J2s of M. graminicola and M. incognita
Heavily infected rice (cv. PB-1121) plants infested with M. graminicola were uprooted from infested plot maintained at the Division of Nematology, ICAR-Indian Agricultural Research Institute, New Delhi. The roots were thoroughly washed and cleaned under tap water and infected roots bearing galls were cut into small pieces. The infected root pieces were kept on muslin cloth, placed on wire mesh (0.5 mm) and kept for a period of 48 h on a petri plate filled with water. The freshly hatched second-stage juveniles (J2s) of M. graminicola emerged from the galls into the water and were collected in a beaker. J2s were counted under the binocular microscope and their numbers were found to be 100+1 mL-1 as an average of 1.0 mL aliquots in triplicates.
Tomato (Solanum lycopersicum) plants of cultivar Pusa Ruby infested with M. incognita and maintained by the Nematology Division, ICAR-Indian Agricultural Research Institute, New Delhi were uprooted and washed. Egg masses were hand-picked using sterilized forceps and transferred to a vial containing sodium hypochlorite (NaOCl -0.5% (v/v)). The egg mass culture was passed through a series of filters (pore size 74, 45, and 25 μm), and eggs were collected in sterile distilled water. The eggs were allowed to hatch in modified Baermann funnels at 28 °C to get J2s for the bioassay. The number of J2s per mL was counted under the binocular microscope and their numbers adjusted to 100+1 per mL, as an average of 1.0 mL aliquot in triplicates.
In-vitro nematicidal bioassay
The nematode suspension (100+1 J2smL-1) was poured into 24-well culture plates and 1.0 mL of different concentrations of test solutions was added, mixed, and incubated at 28 °C. All bioassays were done in triplicate. Polysorbate 80 (Tween-80) (4.0%) in distilled water and sterile distilled water were taken as negative control, while carbofuran 3G and velum prime (Fluopyram 34.48% SC) were used as positive control. The mortality of J2s was recorded after 24, 48, 72, and 96 h of treatment. All dead and alive J2s were counted with the aid of a counting dish under a stereoscopic binocular microscope. The mortality of nematodes was determined by keeping immobile nematodes in fresh distilled water for 24 h and again observed under a stereoscopic binocular microscope. Mortality rates were calculated using Abbott’s formula (Abbott 1925; Rana et al. 2015).
Pot Experiments
Preparation of soil
A soil-sand mixture (3:1) was autoclaved at 1.06 kg per cm3 pressure for 4 h in polythene bags and then cooled and dried in sunlight followed by filling of the soil into formalin (4.0%)-treated earthen-ware pots.
Seedlings
Approximately 500 rice Pusa Basmati (PB-1121) seeds were washed with potassium permanganate solution (1.0%) followed by distilled water and were spread on wetted filter papers in a plastic tray and kept up to 10 days to germinate. Similarly, the seedlings of Solanum melongena cv. ‘Pusa Purple Long’ were raised on sterilized oil from seeds in nursery beds for carrying out pot experiments in rice and brinjal seedlings using root dip and soil drenching methods.
Root dip method
The roots of rice (PB-1121,10 days old) and brinjal (cv. Pusa Purple Long, 30 days old) seedlings were dipped in 50 mL volume of concentrations (1000 and 500 μgmL-1) of mixture of A. squamosa hexane extract (AHE) and A. squamosa methanol extract (AME) from A. squamosa seeds when mixed in equal proportion with citronella oil (CO) or geranium oil (GO) for 30 min. The rice (5 seedlings/pot) and brinjal (2 seedlings pot-1) seedlings were transplanted to the earthen pots containing sterilized soil (1.150 kg pot -1) and kept for 48 h. Freshly hatched J2s (about 2000 J2s pot-1) of M. graminicola and M. incognita were inoculated directly in the root zones of rice and brinjal seedlings after 48 h of transplantation. Total 9 treatments with triplicates were taken in this study.
Soil drenching method
Sterilized soil (1.150 kg) in earthen-ware pots was drenched (50 mL pot-1) separately with two concentrations (1000 and 500 μg mL-1) of herbal mixture of A. squamosa hexane extract (AHE) and A. squamosa methanol extract (AME) from A. squamosa seeds when mixed in equal proportion with citronella oil (CO) or geranium oil (GO) and covered with polythene sheets for 2 days. The ten-day-old rice (5 seedlings/pot) and thirty days old brinjal (2 seedling spot-1) seedlings were transplanted to the earthen pots. The freshly hatched J2s (about 2000 J2s pot-1) of M. graminicola and M. incognita were inoculated directly in the root zones of rice and brinjal seedlings after 48 h of transplantation. A total of 9 treatments with triplicates were taken in this study.
Seedling care and nutrition
Care of seedlings such as watering and application of recommended doses of nitrogenous and phosphoric fertilizers for proper growth of rice and brinjal seedlings together with the random rotation of pots to eliminate the effect of sun and shade were carried out. Also, rice and brinjal seedlings were provided Hoagland’s nutrient solution once a fortnight at the rate of 250 mL pot-1 (Hoagland and Arnon 1950). The observations for rice seedlings’ growth parameters such as root and shoot lengths, fresh root and shoot weights, and the number of galls per seedling in both experiments were recorded on 7, 14, and 30 days after inoculation (DAI). Similarly, root length, shoot length, fresh root weight, shoot weight and number of galls per seedling in both experiments and that of brinjal were recorded in 30 and 60 DAI.
Statistical Analysis
All experiments were performed in triplicate, and the data were subjected to statistical analysis using SPSS (version 22.0) software using one-way analysis of variance (significance level of p < 0.05). The results were presented as mean ± standard deviations.
RESULTS AND DISCUSSION
Chemical Composition of Crude Hexane Extract
Dried and powdered seeds were extracted using hexane. The yield of the hexane extract of A. squamosa was 22.2%, on a dry-weight basis. The crude hexane extract contained mainly total oil and was thus converted into fatty acid methyl esters using methanolic-sulfuric acid (1.0%) to determine its chemical composition. Fatty acids were identified by GC-MS and quantified by GC-FID using its fatty acid methyl esters (Table 1). Eleven fatty acids, accounted for 99.8% of the oil; the most predominant were oleic acid (50.1%), linoleic acid (23.9%), palmitic acid (14.2%), and stearic acid (11.8%) (Fig. 1). GC-MS analysis showed the presence of heneicosanoic acid (2.3%), eicosanoic acid (0.9%), margaric acid (0.2%), 11-eicosanoic acid (0.2%), palmitoleic acid (0.01%), and 17-methyloctadecanoic acid (0.1%).
Table 1. Major Fatty Acid Composition of the Oil of Annona squamosa Seeds
Fig. 1. Gas chromatogram of methyl esters of the fatty oil of Annona squamosa seeds
Nematicidal Activity
In-vitro nematicidal activity
The results of in-vitro nematicidal activity of mixture of A. squamosa hexane extract (AHE) and A. squamosa methanol extract (AME) from A. squamosa seeds when mixed in equal proportion with citronella oil (CO) or geranium oil (GO) against J2S of M. graminicola and M. incognita at a range of concentrations from 31.2 to 1000 μg·mL-1 and period of exposures from 24 h to 96 h showed concentration-dependent mortality of J2s. Mortality of J2s increased with concentration and duration of exposure. The evaluation of nematicidal activity of citronella oil plus A. squamosa methanol plus A. squamosa hexane extract (CO+AHE+AME) mixture against M. graminicola and M. incognita showed that the oil caused mortality of J2 of both nematode species. The results ranged from 33.5 ± 0.6-91.3 ± 1.0 and 66.5 ± 1.3-99.2 ± 1.0% against M. graminicola and 48.5 ± 1.3-88.5 ± 1.3 and 69.7 ± 1.0-99.00 ± 0.8% mortality of M. incognita at 31.2 to 1000 μg mL-1 from 24 h to 96 h of treatment, as listed in Table 2. Lethal concentration (LC50) values were found to be 13.7 μg mL-1 for M. graminicola and 11.6 μg mL-1 for M. incognita after a period of 96 h.
The evaluation of nematicidal activity of geranium oil plus A. squamosa methanol plus A. squamosa hexane extract (GO+ AME+AHE) mixture against M. graminicola and M. incognita showed that the oil caused impressive mortality of J2 of both nematodes, at 31.2 to 1000 μg mL-1 from 24 h to 96 h of treatment (Table 3). LC50 values were found to be 21.8 μg mL-1 for M. graminicola and 8.1 μg mL-1 for M. incognita after a period of 96 h of treatment.
Pot Experiment against M. graminicola
In the pot experiments, a single root dip application of a mixture (CO+AHE+AME) increased rice seedling growth parameters and inhibited formation of galls on roots (Table 4). The mean fresh rice shoot length (32.0 ± 1.0 cm), root length (10.5 ± 0.5 cm), shoot weight (3.2 ± 0.1 g), root weight (1.9 ± 0.1 g), and number of root gall (4.0 ± 0.0) resulted from application of 1000 μg mL-1. Application of 500 μg mL-1 CO+AHE+AME resulted in a decrease in shoot length (27.7 ± 0.6cm), root length (9.0 ± 0.5cm), shoot weight (3.20 ± 0.10g), root weight (1.60 ± 0.10 g), and number of root gall (5.6 ± 0.5) recorded on 30 days after inoculation.
The maximum fresh rice shoot length (30.7 ± 1.5cm), root length (09.7 ± 2.1cm), shoot weight (3.3 ± 0.1 g), and root weight (1.7 ± 0.2 g) were found at 1000 μg mL-1 compared to 500 μg mL-1, in which rice shoot length (30.7 ± 1.5cm), root length (09.0 ± 0.8cm), shoot weight (2.4 ± 0.1 g), and root weight (1.3 ± 0.1g) were recorded on 30 days after inoculation in the GO+AHE+AME mixture. The number of galls after CO+AHE+AME treatment was 4.0 ± 0.0 and 5.67 ± 0.58 galls/seedling, whereas with treatment GO+AHE+AME it was 2.43 ± 0.1 and 3.26 ± 0.1 galls/seedling that were recorded for the 1000 and 500 μg mL-1 treatments, respectively, on 30 days after inoculation, compared to carbofuran (3.0 ± 0.0 and 4.3 ± 0.6 galls/seedling) at concentration of 1000 and 500 μg·mL-1and velum prime (3.6 ± 0.6 and 4.6 ± 0.6 galls/seedling) 1000 μg·mL-1and 500 μg·mL-1. The negative control showed a higher number of galls (14.7 ± 0.6 galls/seedling) during the same period of 30 days. The mixture significantly improved and increased the shoot length and inhibited root galling in the roots of rice seedling (Fig. 2).
However, the use of these mixtures of essential oils (P. graveolens and C. nardus) and hexane extract of seed (A. squamosa) in the management of nematodes in field crops has not been much explored under field conditions or even in greenhouse agriculture and nematicidal products from the mixtures of essential oils and seed extract are not yet developed and available to the farming community.The most effective tested samples have the potential to be used as bionematicides, however, multi-location field trials are suggested to further establish the efficacy under different geographic conditions against M. graminicola and M.incognita in rice and brinjal.
Soil drenching application
Rice seedlings were planted in the soil drenched with mixture of A. squamosa hexane extract (AHE) and A. squamosa methanol extract (AME) mixed in equal proportion with citronella oil (CO) or geranium oil (GO) in pots. In the case of M. graminicola, the results (Table 5) showed a similar effect on seedling growth parameters and root galling but better inhibition of gall formation in the roots of rice than the root dipping method. The number of galls in the CO+AHE+AME treatment was 3.3 ± 0.6 and 5.0 ± 1.0 galls/seedling, and the number of galls in the GO+AHE+AME treatment was 3.7 ± 0.6 and 4.7 ± 0.6 galls/seedling) at 1000 and 500 μg mL-1 treatments vs. carbofuran (2.60 ± 0.6 and 4.0 ± 0.5 galls/seedling) and velum prime (3.0 ± 0.0 and 4.3 ± 0.5 galls/seedling) at 1000 and 500 μgmL-1, which were lesser than the negative control (14.66 ± 0.58 galls/seedling) 30 days after inoculation. The rice seedling growth parameters were found similar in both mixture extract and positive control at 1000 μg mL-1 and were better than the negative control (Fig. 3).
Fig. 2. PB-1121 Root Dipping: Effect of (CO+AHE+AME) and (GO+AHE+AME) on growth parameters and number of galls of Meloidogyne graminicola in roots of rice seedlings in root dip application in pot experiment. *CO-Citronella oil, AHE-A. squamosa hexane extract, AME-A. squamosa methanol extract, GO-Geranium oil
Table 2. In-vitro Nematicidal Activity of Citronella Oil +A. squamosa Hexane Extract (AHE)+ A. squamosa Methanol Extract (AME) on Mortality (%) of J2s of Meloidogyne graminicola and M. incognita
* CO+AHE+AME =Citronella oil + A. squamosa hexane extract (AHE)+ A. squamosa methanol extract (AME), GO+AHE+AME =Geranium oil + A. squamosa hexane extract (AHE)+ A. squamosa methanol extract (AME), C=Carbofuran, V=Velum Prime, WT-80= Water+Tween-80. The values given are the mean ± standard deviation of three replicates.
Table 3. In-vitro Nematicidal Activity of Geranium Oil +A. squamosa Hexane Extract (AHE)+ A. squamosa Methanol Extract (AME) on Mortality of J2s of Meloidogyne graminicola and M. incognita
* GO+AHE+AME =Graminicola oil + A. squamosa hexane extract (AHE)+ A. squamosa methanol extract (AME), GO+AHE+AME =Geranium oil + A. squamosa hexane extract (AHE)+ A. squamosa methanol extract (AME), C=Carbofuran, V=Velum Prime, WT-80= Water+Tween-80. The values given are the mean ± standard deviation of three replicates
Table 4. Effect of CO+AHE+AME and GO+AHE+AME on Growth Parameters and Number of Galls of Meloidogyne graminicola in Roots of Rice Seedlings in Root Dip Application in Pot Experiment