AbstractNorway spruce (Picea abies [L.] Karst.) is an important forest species, comprising approximately 55.9% of the growing stock of Czech forests. The variations in the wood densities from three different locations were evaluated with respect to their mechanical and physical properties. Also, mechanical properties and formaldehyde emission of particleboard (PB) and medium-density fibreboard (MDF) panels produced from Norway spruce wood were investigated. The overall average density of the spruce wood was 509.22 kg/m3, ranging from 400.95 ± 27.92 to 617.50 ± 29.91 kg/m3 by location. Most of the panels exceeded the requirements of EN standards for the measurements of MOE, MOR, and the internal bond. Furthermore, the results showed highly significant differences (p < 0.001) among the panels for PB and MDF, which could be related to inter-panel variations. The formaldehyde emissions of PB and MDF were below the E1 emission limits. Moreover, positive correlations were found between the formaldehyde emissions (perforator and gas analysis methods) and board density. The results of this study verify our knowledge of wood density variation as affected by location as well as the age of trees and their relationship to mechanical and physical properties. Consequently, the variation in mechanical properties of the produced panels as well as the formaldehyde emission can further contribute to creating models to predict the quality of the product.