In the European Union, timber is used in structural applications and must be graded with a Conformité Européene (CE) mark. To achieve standard, machine strength grading is used. A common technology for these machines is based on using the vibrational response of each wood board to estimate the timber modulus of elasticity and modulus of rupture. The first Eigen frequency is usually used to predict these mechanical properties. However, in heterogeneous wood species such as oak, this parameter is less correlated with mechanical properties. The current study proposes two new methods based on an extended exploitation of the vibrational response that predicts oak wood mechanical properties. The first method was based on the mechanical parameters deduced from several Eigen frequencies that were chosen with regards to a stepwise regression. The second method was based on the full vibrational spectrum and used a partial least squares method. The first method slightly improved the prediction of the modulus of elasticity compared with the first Eigen frequency in edgewise transversal vibration. Both methods significantly improved the prediction of the modulus of rupture.