NC State
Liu, H., Wang, L., Sun, Z., Pang, L., Jia, M., Wu, S., Sun, X., Hao, L., Gao, S., Jia, S., and Jia, L. (2012). "Production and antioxidant activity of intracellular polysaccharide by Hypsizigus marmoreus SK-01," BioRes. 7(4), 5879-5893.


The extraction parameters of intracellular polysaccharide (IPS) from Hypsizigus marmoreus SK-01 mycelia were optimized, and the antioxidant activities of IPS were investigated. The optimum conditions of IPS extraction were predicted to be: an ultrasonic treatment time of 618.07 s, an extraction temperature of 84.53 °C, a pH of 7.57, and an IPS yield of ca. 6.84%. The in vitro inhibition effects of IPS on hydroxyl, superoxide anion, and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 52.63 ± 3.19%, 68.21 ± 5.09%, and 63.43 ± 5.27%, respectively, and the EC50 values of IPS were 0.85 ± 0.05 g/L, 0.47 ± 0.03 g/L, and 0.62 ± 0.04 g/L, respectively. The activities of glutathione peroxidase (GSH-Px), superoxide dismutase (SOD), and catalase (CAT) in mice blood were 2.52 ± 0.02 U/mL, 453.19 ± 38.43 U/mL, and 118.59 ± 9.64 U/mL, respectively. The results provide a reference for large-scale extraction of IPS by H. marmoreus SK-01 in industrial fermentation, suggesting that IPS can be used as a potential antioxidant that enhances adaptive immune responses.
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