AbstractFerulic acid is present at relatively high concentrations in the cell walls of several plants. Agricultural lignocelluloses are now used as bioresources in industry. This study attempted to increase the free ferulic acid content present in lignocellulose by using thermostable esterase produced from thermophilic actinomycetes to hydrolyze ester bonds. Destarched wheat bran was used as a carbon source for the production of esterases from the newly isolated thermophilic actinomycetes species Thermobifida fusca. After 96 h of cultivation, the esterase activity that accumulated in the culture broth was 946.0 U/mL. Two percent of the destarched wheat bran was then hydrolyzed by crude esterase preparation for 16 h. The ferulic acid was accumulated in the culture broth at a concentration of 310.0 mM. The hydrolysate had better radical-scavenging ability for both 1,1-diphenyl-2-picrylhydrazyl and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) radical-scavenging ability, as well as reducing power than ferulic acid. These results showed that the ferulic acid-rich destarched wheat bran hydrolysate had good antioxidant properties. It is suggested that this process can be advantageous for the industrial production of antioxidants derived from agricultural bioresources.