AbstractThe aim of the paper was to examine the application of regenerated cellulose fibers as a reinforcement material in particleboard production. Single-layer, 10 mm thick panels, with the density of 800 kg/m3 were produced with addition of regenerated cellulose fibers in the range of 0 do 15% by mass during panels’ production. The mechanical and physical parameters of the produced panels were tested, as well as work of fracture. The results showed that addition of regenerated cellulose fibers to the structure of wood-based composite did not improve their modulus of rupture, modulus of elasticity, or internal bond. The physical parameters of the produced panels (water absorptivity, swelling in thickness when soaking) also were reduced. The work of fracture of the tested panels increased with increasing content of regenerated cellulose fibers. A strong linear regression between work of fracture and regenerated cellulose fibers content was observed.