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Bukhari, N. A., Jahim, J.M., Loh, S. K., Nasrin, A. B., and Indera Luthfi, A. A. (2019). "Response surface optimisation of enzymatically hydrolysed and dilute acid pretreated oil palm trunk bagasse for succinic acid production," BioRes. 14(1), 1679-1693.

Abstract

The exploitation of agroindustrial lignocellulose, such as oil palm trunk bagasse (OPTB), as a raw material in the production of succinic acid (SA) may serve as an effective strategy to propel the bio-based industry. This study aimed to optimise the recovery of fermentable sugar, i.e., glucose, from enzymatic hydrolysis of the dilute acid pretreated OPTB (DA-OPTB). The dilute acid pretreatment used in this study was able to remove 59.5% of hemicellulose and 13.3% of lignin. Response surface methodology (RSM) based on central composite design (CCD) was then applied to investigate four independent variables – enzyme loading (10 to 50 U/g), agitation speed (50 to 250 rpm), reaction time (0 to 96 h), and surfactant concentration (0.025 to 0.125%, v/v). The experimental glucose concentration of 21.7 g/L was in good agreement with the RSM-predicted value of 20.5 g/L. Among the parameters investigated, supplementation of a surfactant during enzymatic hydrolysis was significant in influencing glucose recovery, while the extent of the agitation speed was the least influential. The maximum recovered glucose was estimated at 217 g per kg of raw OPTB, with 7.3 g/L of SA attainable from the fermented DA-OPTB hydrolysate using Actinobacillus succinogenes 130Z. The results demonstrated that OPTB can be practically utilised in the economical production of high value-added SA.


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