Soft donors of nitrogen and sulfur were incorporated into enzymatic hydrolysis lignin (EHL-NS) to make it suitable for multiple applications. Characterizations of the environmentally friendly material by Fourier transform infrared spectroscopy, scanning electron microscopy, energy dispersive spectroscopy, and thermogravimetric analysis confirmed the layered porous structure with nitrogen and sulfur groups. It exhibited high antioxidant activity due to the strong electron-donating capability of the soft donors. Moreover, the soft donors also contributed to the chemical complexation of Hg(II) with EHL-NS, which distinctly enhanced the adsorption of Hg(II) in water (Qe=180 mg/g, 25 °C). Given that the free radicals were highly effective at scavenging and adsorption, the functionalized enzymatic hydrolysis lignin is expected to serve a useful role.