This study investigated two classifications of wood cellulose of particle sizes 300 µm to 424 µm and 600 µm to 849 µm. The cellulose samples were characterized using X-ray diffraction (XRD), energy-dispersive X-ray spectroscopy (EDX), and scanning electron microscopy (SEM). The cellulose crystal revealed a preferred orientation along the (200) plane for the most prominent peak. The XRD diffractogram revealed an orthorhombic structure obtained from the powder diffractogram file (PDF). Furthermore, the crystallinity index and crystalline size were calculated and the increase in crystalline size of the isolated cellulose indicated higher thermal stability. The EDX analysis showed chemical components of carbon (C), sodium (Na), chlorine (Cl), and oxygen (O) in the isolated cellulose. The morphology of the cellulose appeared as strings of fibres. The isolated cellulose has applications in the production of biomaterial, thermal insulating devices, and domestic applications.