AbstractNutrients were extracted from corn stalks, peanut shells, de-oiled peanut meal, chicken manure, and sewage sludge by a subcritical water (SCW) hydrolysis reaction. Compared with the other feedstock, the aqueous phases extracted from de-oiled peanut meal showed the highest water-soluble organic carbon, amino acid, total nitrogen, and phosphorus contents. The effects of solution pH, final hydrothermal temperature, and reaction time on nutrient extraction from de-oiled peanut meal were investigated. The analysis showed that alkaline reagents promoted liquefaction. The highest yield of the total primary nutrients (82.6%) was obtained with extraction reaction at 180 °C for 1.5 h using 0.1 mol/L KOH. The liquid fraction from this reaction was investigated for its potential use as a fertilizer with germination experiments. A higher germination index and root activity were obtained using the liquid extract with the appropriate dilution. These results indicated that subcritical water hydrothermal treatment is a viable way to recover nutrients from biomass wastes. In addition, de-oiled peanut meal is a suitable feedstock for the production of nutrient-rich liquid extract.