The effect of laboratory refining on de-inking potential of inkjet printed handsheets was investigated. Pulp samples containing 80% short fiber and 20% long fiber were beaten in a PFI mill to reach four predetermined freeness levels of 650 (unrefined), 550, 430, and 340 mL CSF, and then handsheets were made. Handsheets were identically inkjet printed and then de-inked. Results revealed that, at lower freeness value, the brightness of de-inked pulps was higher, but the opacity decreased. The surface roughness of handsheets produced using different refined pulp before de-inking was reduced. Our results showed that refining will impart a positive effect on handsheets’ de-inking potential, and de-inking printed papers produced from pulps refined to lower freeness generated the highest brightness. The results revealed that both tensile and tear strength indices of de-inked pulp were lower. However, the tear strength index of unrefined sample and the tensile strength index of pulp refined to 430 ml CSF were higher than for undeinked samples.