Abstract
To improve the nematode-infected pinewood application value, an effective strategy was developed based on the cultivation of Flammulina velutipes to process the pinewood that was infected by the nematode Bursaphelenchus xylophilus. Different treatments were compared to determine the optimal method to obtain the highest yields of reducing sugars. The results showed that the cultivation of F. velutipes using the nematode-infected pine sawdust overcame the disadvantage of cultivation by using healthy pine sawdust, and whether fungal infection that happened in the period of pine wilt disease or during the cultivation of F. velutipes both can contribute on the degradation of the polysaccharide content. A high yield of reducing sugars can be obtained using 2% dilute sulfuric acid at 121 °C for 1 h to treat recyclable nematode-infected pine sawdust after the cultivation of F. velutipes. Additionally, the results showed that fungal infection that occurred in the period of the pine wilt disease and during the cultivation of F. velutipes in addition to acid hydrolysis effectively converted hemicellulose to reducing sugars.
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Utilization of Nematode-infected Pinewood for Mushroom Cultivation and Production of Reducing Sugar
ChenYan Huang,a,1 Xuan Wu,a,b,1 XiaoYi Dai,a Rebeca Carballar-Lejarazú,c YongXin Tao,d JianRong Wen,e Ren Chen,f FeiPing Zhang,a,* and SongQing Wu a,*
To improve the nematode-infected pinewood application value, an effective strategy was developed based on the cultivation of Flammulina velutipes to process the pinewood that was infected by the nematode Bursaphelenchus xylophilus. Different treatments were compared to determine the optimal method to obtain the highest yields of reducing sugars. The results showed that the cultivation of F. velutipes using the nematode-infected pine sawdust overcame the disadvantage of cultivation by using healthy pine sawdust, and whether fungal infection that happened in the period of pine wilt disease or during the cultivation of F. velutipes both can contribute on the degradation of the polysaccharide content. A high yield of reducing sugars can be obtained using 2% dilute sulfuric acid at 121 °C for 1 h to treat recyclable nematode-infected pine sawdust after the cultivation of F. velutipes. Additionally, the results showed that fungal infection that occurred in the period of the pine wilt disease and during the cultivation of F. velutipes in addition to acid hydrolysis effectively converted hemicellulose to reducing sugars.
Keywords: Nematode-infected pinewood; Flammulina velutipes cultivation; Acid hydrolysis; Reducing sugars
Contact information: a: Key Laboratory of Integrated Pest Management in Ecological Forest, Fujian Province University, College of Forestry, Fujian Agricultural and Forestry University, Fuzhou, 350002, China; b: College of Environment and Ecology, Xiamen University, Xiamen, 361000, China; c: Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, CA 92697 USA; d: China National Engineering Research Center of JUNCAO Technology, Fujian Agriculture and Forestry University, Fuzhou, 350002, China; e: Longyan Customs, Longyan, 364000, China; f: Putian Entry-exit Inspection and Quarantine Bureau, Putian, 351100, China; 1: These authors contributed equally to this work; *Corresponding author: fpzhang1@163.com, dabinyang@126.com
INTRODUCTION
The pine wilt disease is caused by the Bursaphelenchus xylophilus nematode. This devastating disease results in a large area of pine trees (Pinus massoniana) dying. There are more than one million trees of P. massoniana that have been infected since 1982 (Shi et al. 2008). Pine wilt disease has a strong transmission and destruction index, which leads to millions in economic losses. Currently, the main direct and effective strategy to control the pine wilt disease is to discover the infected pine trees in real-time that are felled immediately to prevent the dispersion of the disease to the surrounding healthy trees. However, the lack of proper treatment of the felled trees can cause a secondary transmission of the disease (Jiang et al. 2001). Therefore, it is important to find an effective method to deal with a large number of felled infected pine trees and prevent a secondary spreading.
Currently, the main remediation methods for the nematode-infected wood are fumigation, local incineration, heat, and microwave treatment (Song et al. 1994; Jiang et al. 2006; Gan et al. 2010). Although these remediation methods are effective and can reduce some losses, they also cause serious pollution effects on the environment and consume a lot of energy. In contrast, other methods are environmentally friendly, such as wood rot bacteria decomposition (Chen et al. 2008) and using the nematode-infected pinewood as a substrate to cultivate Poria cocos (Wu 2013). This last method suggests that the nematode-infected pinewood can be used for edible fungus cultivation. Moreover, sawdust from the nematode-infected pinewood is abundant in cellulose and hemicellulose and it can be used as a substrate to grow edible filamentous fungus (Lu et al. 2016). Flammulina velutipes is one of the main edible fungi with high nutritional and medicinal value (Yu et al. 2004). However, healthy pine sawdust cannot be directly used to cultivate the F. velutipes due to the effect the pine wood terpenoids (Chen et al. 2002). Alternatively, cultivation of F. velutipes by using nematode-infected pinewood is feasible because the terpenoids content of the nematode-infected pinewood is lower than in the healthy pinewood (Kuroda 1989). If the nematode-infected pine sawdust is used for the cultivation of F. velutipes, it can effectively prevent the spread of B. xylophilus and greatly improve the utilization rate of nematode-infected pinewood and help to reduce the cost of F. velutipes cultivation.
While F. velutipes cultivation can effectively improve the utilization rate of the nematode-infected pine sawdust, in contrast, the spent mushroom substrate will still have a certain impact on the environment. If the efficient recycling mechanism of nematode-infected pine sawdust can be established, it will be beneficial to overcome the disadvantages of traditional methods and gain new profits. It has been reported that reducing sugar can be obtained using the biomass conversion method to treat spent mushroom substrate (Wu et al. 2013). The method can degrade lignin or hemicellulose on the surface of the material to realize the conversion of cellulose into reducing sugar, thereby increasing the yield of reducing sugar. Therefore, finding the suitable conditions for the conversion of nematode-infected pine sawdust into reducing sugar will contribute in developing a sustainable method to treat nematode-infected pinewood based on the re-utilization of the infected materials.
In this study the authors used broad-leaved wood sawdust, healthy pine sawdust, and nematode-infected pine sawdust to cultivate F. velutipes, and the feasibility of growing F. velutipes was evaluated by comparing the yield of F. velutipes. The reducing sugar yield was analyzed by using different material sources (healthy pine sawdust and nematode-infected pine sawdust, healthy pine substrate, and nematode-infected pine substrate) under different treatments (acid, alkali, and water treatment). The healthy pine substrate and nematode-infected pine substrate refer to a type of spent mushroom substrate. The healthy pine and nematode-infected pine substrates are made from using the healthy pine sawdust and nematode-infected pine sawdust to cultivate F. velutipes, respectively. The treatment efficiency was determined using scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier transforming spectroscopy (FTIR), and X-ray photoelectron spectroscopy (XPS) to analyze the morphological, physical, and chemical structures of the sample surface under the same treatment conditions. The data provide insight into a more economical and environmentally friendly method and a high-efficiency recycling process for the use of the nematode-infected pinewood.
EXPERIMENTAL
Materials
Cultivation of F. velutipes
Infected pinewood and healthy pinewood were both collected from Minhou County, Fuzhou City (Fujian Province, China). All nematode-infected pinewood samples used in this research had been previously ground. The substrate ingredients for growing F. velutipes contain 58% cottonseed shell, 20% wood sawdust, 20% bran, 1% gypsum, and 1% sucrose. The dry material weight was 270 g for each sample (broad-leaved wood sawdust, healthy pine sawdust, and nematode-infected pine sawdust); 90 g of each material was put in bottles in triplicate. The bottles containing the materials were sterilized (121 °C, 4 h, 103.4 KPa) and then inoculated with F. velutipes FL19 and cultivated at 25 °C. After the mycelium grew and covered the material, it was kept in culture at 10 °C and at 80 to 90% humidity until the harvesting time (stipe length of 15 + 2.5 cm and a cap diameter of 0.9 ± 0.2 cm). The fresh weight of F. velutipes was recorded immediately after harvest. The average mushroom yield of each bottle was recorded and analyzed. Finally, the spent mushroom substrate was recycled for later experiments.
Methods
Different hydrolysis treatment and composition detection
After air drying (60 °C, 72 h), each substrate (healthy pine sawdust, nematode-infected pine sawdust, healthy pine substrate, and nematode-infected pine substrate) was milled with a grinder to pass through a 40-mesh screen (425 um). Different treatment conditions were set using different chemicals, concentrations, and temperature to determine the optimal conditions that resulted in higher yields. The reaction conditions included acid hydrolysis (concentration 0.5 to 4% and 50 to 121 ℃, 1 h), alkaline hydrolysis (concentration 1%, 121 ℃, 1 h), and water treatment (121 ℃, 1 h) (Wu et al. 2013). Reactions were performed by adding 5 g of the substrate to each chemical at 1:10 ratio (solid : liquid) in a 250-mL conical flask. After the above reaction was finished, the mixed solution was allowed to cool, followed by the addition of the corresponding concentration of H2SO4 or NaOH to neutralize the mixture solution (pH = 7) and stop the reaction. The neutralized samples were washed four times with distilled water followed by centrifugation (8000 r/min, 4 °C, 5 min/time). The solid residues were collected and put into an oven at 45 °C to dry and seal the substrate surface for subsequent experiments. The supernatant was collected for chemical composition analysis and to determine the yield of reducing sugar. The moisture and ash contents of the samples were determined according to the People’s Republic of China national standard GB/T 2677.2 (2011) and GB/T 742 (2008), respectively. The contents of cellulose, hemicellulose, acid-soluble lignin (ASL), and acid-insoluble lignin (AIL) were determined according to the National Renewable Energy Laboratory (NREL) analytical procedure (Sluiter et al. 2008). Each sample was performed in triplicate. The content of reducing sugar was determined by high-performance liquid chromatography (HPLC) (Agilent 1260; Agilent Technologies, Shanghai, China) with an Agilent Zorbax SB-C 18 column (4.6 mm × 250 mm, 5 μm). Chromatographic conditions were 16% acetonitrile to 84% 0.05 mol/L ammonium acetate solution and in a wavelength of 245 nm, with a column temperature of 30 °C and a flow rate of 1.5 mL/min.
SEM analysis
The morphology surface of the solid residues was analyzed by using SEM, field emission SU8010 (Hitachi High-Technologies, Tokyo, Japan). The dried solid powder was put on the clean silicon wafer, and then the metal (gold) spray was used to make the sample detectable (S. S. Aerosols Pvt. Ltd., Haryana, India). Finally, the image was scanned and saved using the SEM software (Microscope Control v5.2.2 build 2898, Hitachi High-Technologies, Tokyo, Japan).
XRD analysis
Structure surface changes of the samples were determined by X-ray diffraction. The detection instrument was a Rigaku Ultima IVX XRD (Tokyo, Japan). The sample was dried and added to a clean vessel and then placed on the instrument. The Cu Kα sample parameter was obtained at 40 kV and 40 mA from a Bragg angle (2θ) of 5° to 60° with a step size of 0.02° and scanning rate of 6°/min. The crystallinity index (CrI) of initial and treated samples was calculated according to the protocol already described. The CrI was determined using the following Eq. 1,
(1)
where CrI is the cellulose crystallinity index (%), I002 is the adsorption peak intensity of peak 002 at 2θ of 22.3, and Iam is the minimum intensity height between peaks 002 and 101 at 2θ of 18°.
FTIR Analysis
Changes in the chemical composition of the sample surface were analyzed by FTIR. The dried samples were mixed with potassium bromide (mass ratio 1:100). The samples were ground into a powder and then pressurized, followed by analysis with a Nicolet iS10 (Thermo Fisher Scientific, San Diego, CA, USA) with a scanning spectrum range of 400 to 4000 cm-1.
XPS Analysis
Changes in the content of the main components (polysaccharide, lignin, and surface extracts) were analyzed by XPS. The instrument used was an Escalab 250Xi (Thermo Fisher Scientific, San Diego, CA, USA). The analytical area of the sample was 500 μm × 500 μm, and the analytical depth was less than 10 nm. The vacuum degree of the analysis was 1 × 10-10 mbar. To weaken the effect of binding energy on the XPS test, the C1s peak was set to 284.8 eV to correct the charge. The raw data of the XPS test were processed using the XPSPEAK 4.1 and OriginPro 8 software (Microsoft Corporation, Redmond, WA, USA).
Statistical Analysis
After the sample treatment and analysis, the samples of each different treatment condition were performed in triplicate, and the data were expressed as mean ± standard deviation. The analysis of variance (ANOVA) analysis PRISIS 6 (GraphPad software, La Jolla, CA, USA) and SPSS statistical software (Version 19.0, IBM Corp., Armonk, NY, USA) were used to analyze the significance of the difference, and the result, P < 0.05, showed that there were significant differences corresponding to different experimental conditions.
RESULTS AND DISCUSSION
Comparative Analysis of F. velutipes Yield
To determine the feasibility of cultivating F. velutipes on nematode-infected pine sawdust, the yield of F. velutipes was compared and analyzed on three different kinds of wood sawdust (broad-leaf wood sawdust, healthy pine sawdust, and nematode-infected pine sawdust). Cultivation of F. velutipes on broad-leaf wood sawdust takes 27 days, and 31 days was taken when cultivated on healthy or nematode-infected pine sawdust (Table 1). However, the mycelial growth was sparse when F. velutipes was cultivated on healthy pine sawdust. Interestingly, F. velutipes yields were different among the substrates, the average yield per bottle was 39.7 g on broad-leaved wood sawdust, 38.5 g on nematode-infected pine sawdust, and 28.6 g on healthy pine sawdust (Table 1). The observed differences may be because the growth of hyphae can be affected by the number of terpenoids during its cultivation on healthy pine sawdust. In contrast, the yield of F. velutipes cultivated in nematode-infected pine sawdust was not significantly different from that cultivated on broad-leaved wood, because the terpenoids can be volatilized more easily after the pinewood was infected by nematode (Kuroda 1989). These results suggest that nematode-infected pine sawdust is suitable for the cultivation of F. velutipes and can help to improve the economic value of nematode-infected pinewood.
Table 1. Mycelium Growth and Mushroom Emergence Period on Various Substrate Materials
Chemical Composition Analysis
The chemical components of the different substrates under different treatment conditions were determined and compared with some of the most common agricultural wastes. The percentage of polysaccharide (including cellulose and hemicellulose) content in healthy pine sawdust was 54.2%; however, it was lower in nematode-infected pine sawdust (51.5%), healthy pine substrate (48.4%), and nematode-infected pine substrate (50.2%). In addition, the percentage of lignin content of nematode-infected pine sawdust (29.8%) was also lower than that of healthy pine sawdust (34.1%). It can be inferred from the results that the differences were due to the hydrolysis of some cellulose and hemicellulose that occurred during the period of infection by the pinewood nematode and the cultivation of F. velutipes, and the hydrolysis may have been caused by the fungal infection. According to Lara et al. (2010) the pine bark composition can be easily digested by fungus. Moreover, some other studies reported that blue-stain fungi often can be found in the nematode-infected pinewood during the period of the pine wilt disease (Mamiya 1983; Song 1993). In contrast, F. velutipes is a fungus, and its cultivation on wood to some degree means that the wood is infected by fungi (Zhao et al. 2017).
Table 2. Chemical Composition of Pine Wood Before and After Treatment, and Comparison with Some Agricultural Wastes
The fungal hydrolysis that happened in the process of F. velutipes cultivation could lead to a lower percentage of polysaccharide content in the healthy pine substrate (48.4%) when compared with the healthy pine sawdust (54.2%). The lower lignin content percentage is helpful to reduce the blocking effect of the dense structure on the surface of the material and improve the conversion rate of the polysaccharide content (Zheng et al. 2018). However, the percentage of lignin content in the healthy pine substrate (37.2%) was higher than that of healthy pine sawdust (34.1%) due to the partial digestion of the polysaccharide content during the cultivation of F. velutipes, which indirectly increased the proportion of lignin content in the material. In addition, the percentage of polysaccharide content in the four kinds of materials (54.2%, 51.4%, 48.4%, and 50.2%) was higher than that of the common agricultural wastes such as corn straw (37.7%) (Chen et al. 2016) and distiller’s grains (41.6%) (Zheng et al. 2015). Therefore, all four materials might have great potential to produce reducing sugar.
Comparison of Reducing Sugar Production
To find the optimal treatment conditions for reducing sugar conversion, this study used HPLC to analyze the yield of reducing sugar under different treatment conditions. As shown in Table 3, the alkali-treated reducing sugar content was not detectable. The reason may be that sodium hydroxide reacts chemically with the monosaccharides hydrolyzed by cellulose, resulting in a decrease in the yield of reducing sugars under high-temperature conditions (Zheng et al. 2015). The yield of reducing sugar after hot water treatment was also unsatisfactory. Only healthy pine sawdust (4.8 g/kg) and nematode-infected pine sawdust (13.5 g/kg) produced a small amount of reducing sugar. This may be due to the degradation of a small portion of the surface hemicellulose or cellulose (Pérez et al. 2008). Therefore, although water treatment has the advantages of low cost and no corrosion, it is not efficient enough for transforming woody cellulose.
Among the treatments, acid treatment showed a higher yield of reducing sugar due to the degradation of most of the hemicellulose; these results are similar to those in Salvinia molesta (Syaichurrozi et al. 2018). The yield of reducing sugar in all the substrates increased with the reaction temperature and acid concentration, especially at 121 °C with 4% H2SO4 for 1 h. The highest reducing sugar yield was obtained for healthy pine sawdust, nematode-infected pine sawdust, and healthy and nematode-infected pine substrate with 111.9 g/kg, 236.8 g/kg, 101.3 g/kg, and 137.2 g/kg, respectively. However, considering the cost of the reagents, instruments, and wastewater treatment costs, the most economical and effective treatment was 121 °C with 2% H2SO4 for 1 h. In this condition, it was found that the yield of reducing sugar from healthy (97.7g/kg) and nematode-infected pine substrate (126.3 g/kg) were not higher than that of nematode-infected pine sawdust (202.2 g/kg), probably because the polysaccharide content of nematode-infected pine substrate was digested by the fungi during the period of the infection by pinewood nematode. In addition, the polysaccharide content of healthy and nematode-infected pine substrate was digested by F. velutipes, resulting in a decrease in the yield of reducing sugar. However, the yield of reducing sugar from the nematode-infected pine substrate (126.3 g/kg) was still higher than that obtained from the healthy pine sawdust (98.6 g/kg) after acid hydrolysis. The results show that the nematode-infected pine substrate still has a great utilization value due to its capacity to be recycled to produce reducing sugar. Moreover, the obtained reducing sugar can be used as a cost-effective carbon source for cultivating microorganisms being of great benefit not only for the biopesticide manufacture but also for reducing agricultural waste (Wu et al. 2013; Zheng et al. 2015, 2018).
Table 3. Yield of Reducing Sugar Under Different Treatment
SEM Analysis
It is well known that the nematode-infected pinewood can be infected also by fungi during the pine wilt disease (Mamiya 1983; Song 1993), and while the pinewood sawdust was used to cultivate F. velutipes, it will also be infected by fungi. To determine the effect of fungal infection during the period of pine wilt disease and its impact on mushroom cultivation and on the cell wall structure of the substrate, the morphology of the raw and the treated materials were analyzed by SEM. The surface structure of the raw healthy pine sawdust was smooth and compact; however, a rough and disordered surface appeared in the raw nematode-infected pine sawdust, as wells as the healthy and the nematode-infected pine substrate (Fig. 1). It can be observed that the fungus infection during the period of pine wilt disease and mushroom cultivation all can promote the hydrolysis of polysaccharide and lignin and destroy the original dense structure of the healthy pinewood under the optimal acid treatment (121 ℃, 2% H2SO4, and 1 h). The surface of the four materials was disintegrated and contained a large number of holes and deep cracks as a result of a large amount of hydrolyzed hemicellulose. Chen et al. (2016) determined that the acid treatment of maize straw produced similar results. In addition, the structure of nematode-infected pine sawdust, healthy pine substrate, and nematode-infected pine substrate were more damaged when compared with healthy pine sawdust, probably due to the fact that the surface structure of healthy and nematode-infected pine substrate is pre-digested as a result of the infection, which makes them more sensitive to acid hydrolysis (Alexandropoulou et al. 2016).
Fig. 1. SEM images of raw and treated materials: A: raw healthy pine sawdust, B: healthy pine sawdust with acid treatment, C: raw healthy pine substrate, D: healthy pine substrate with acid treatment, E: raw nematode-infected pine sawdust, F: nematode-infected pine with acid treatment, G: raw nematode-infected pine, H: nematode-infected pine substrate with acid treatment; acid treatment: 121 °C, 2% H2SO4, and 1 h
Studies have shown that highly loose and chaotic surfaces contribute to the degradation of more quantities of cellulose and hemicellulose (Auxenfans et al. 2017). However, although the destruction degree of the surface structure of the nematode-infected pine substrate was higher than that observed from the nematode-infected pine sawdust, the yield of reducing sugar was the opposite due to a pre-degradation of the cellulose and hemicellulose during the cultivation of edible fungi which is consistent with previous studies (Chen et al. 2002).
XRD Analysis
Crystallinity is an important factor to demonstrate the compact structure of the material surface. In this study, the crystallinity of the four substrates was analyzed by XRD before and after the treatment. The strengths of the crystalline region (002) and the non-crystallized region (am) of the sample after fungal infection and acid hydrolysis were different (Fig. 2). The percentage of crystallinity in the healthy pine sawdust, healthy pine substrate, nematode-infected pine sawdust, and nematode-infected pine substrate was 51.5%, 49.6%, 48.1%, and 50.1%, respectively (Table 4). The crystallinity from the other three materials decreased slightly when compared with the crystallinity from the raw healthy pine sawdust. A possible explanation is that some cellulose degradation may occur in some crystalline regions during fungal infection (Sánchez 2009). After the acid hydrolysis (121 ℃, 2% H2SO4, and 1 h), the percentage of crystallinity in the healthy pine sawdust, healthy pine substrate, nematode-infected pine sawdust, and nematode-infected pine substrate increased to 62.1%, 60.5%, 56.8%, and 58.1%, respectively (Table 4). These results show that acid hydrolysis can effectively remove the xylan from the materials and then destroy the surface structure of the hemicellulose and lignin promoting more cellulose to be exposed to the acidic solution and finally converted into reducing sugar (Li et al. 2016). However, the increase of crystallinity from the nematode-infected pine sawdust and the nematode-infected pine substrate was smaller after acid hydrolysis, which may result from the fact that some of the biodegradable non-crystalline cellulose is digested during fungal infection.
Fig. 2. XRD curves of raw and treated materials: A: raw nematode-infected pine sawdust, B: raw healthy pine substrate, C: raw nematode-infected pine substrate, D: raw healthy pine sawdust, E: nematode-infected pine with acid treatment, F: nematode-infected pine substrate with acid treatment, G: healthy pine substrate with acid treatment, H: healthy pine sawdust with acid treatment; acid treatment: 121 °C, 2% H2SO4, and 1 h
Table 4. Crystallinity of Raw and Acid Treated Substrates
FTIR Analysis
To determine the changes in the chemical structure of the substrates, FTIR was used to analyze the characteristic functional groups of the component surface (cellulose, lignin, and hemicellulose) before and after the sample treatment. The absorption spectra are shown in Fig. 3. The change in the absorption peak strength after the treatment is related to the change of the corresponding lignocellulose composition.
The absorption at 1458 cm-1 is related to the deformation of CH3 in the lignin, and the stretching vibrations of C = C in the lignin are indicated by an absorptive peak 1509 cm-1. The absorption peak intensity from the healthy pine substrate, nematode-infected pine substrate, and nematode-infected pine sawdust at 1458 cm-1 and 1509 cm-1 was lower than in the healthy pine sawdust before the acid hydrolysis (Fig. 3), indicating that the fungal infection caused by pine wilt disease or the edible fungus could hydrolyze lignin content. Whereas acid hydrolysis can further degrade lignin content (Jordan et al. 2008), the absorption peak intensity of all samples decreased in the treated materials when compared with the untreated materials.
The absorptions at 1245 cm-1 and 1737 cm-1 are related to the alkyl ester of the acetyl group and non-conjugate stretching of C = O in the hemicellulose, respectively. It can be seen that the peaks significantly decreased after fungal infection or acid hydrolysis compared with the peaks of raw healthy pine sawdust and raw healthy pine substrate (Fig. 3), suggesting that both fungal infection and acid treatment can effectively hydrolyze hemicellulose. It is worth noting that the absorption peak intensity of nematode-infected pine substrate and healthy pine substrate after acid hydrolysis was lower than that of nematode-infected pine sawdust after acid hydrolysis (Fig. 3), which indicates that hemicellulose in the sample of fungal infection combined with acid hydrolysis can be used more effectively.
The broad peak at 3000 cm-1 to 3500 cm-1 and the absorbance at 898 cm-1 represents the O-H stretching and aliphatic or aromatic C-H bonds, respectively. Compared with the raw healthy pine sawdust, the peak intensity of the nematode-infected pine sawdust, the healthy, and the nematode-infected pine substrate was decreased (Fig. 3), suggesting that the cellulose content of the digestible part of the fungal infection was consistent with the results of the previous component analysis. The peak strength of the four materials after acid hydrolysis decreased slightly at 3000 cm-1 to 3500 cm-1 and at 898 cm-1 (Fig. 3), suggesting that some cellulose contents were hydrolyzed during acid treatment, but the ability of acid to degrade cellulose was limited.
The absorptions at 2850 cm-1 and 2920 cm-1 are related to the stretching of the CH2 group on the surface of fatty extracts. The peak intensity of nematode-infected and healthy pine substrate was higher than corresponding raw materials (Fig. 3), which may have been because fungal infection decreased the proportion of surface extracts indirectly by decreasing the lignocellulose components. However, the peak strength of all samples after acid hydrolysis did not change significantly (Fig. 3), indicating that acid hydrolysis cannot remove fat extracts.