NC State
M.S. Khan, J. Tian, L. Xu, W. Shen and G. Garnier. Bioactive enzymatic papers. In Advances in Pulp and Paper Research, Oxford 2009, Trans. of the XIVth Fund. Res. Symp. Oxford, 2009, (S.J. I’Anson, ed.), pp 1149–1166, FRC, Manchester, 2018.


The thermal stability of Alkaline phosphatase enzyme (ALP) adsorbed on paper was measured using a colorimetric technique quantifying the intensity of the product complex. ALP adsorbed on paper retains its functionality and selectivity. Adsorption of ALP on paper increased the enzyme thermal stability by 2 to 4 orders of magnitude compared to the same enzyme in solution. Complex patterns of enzyme were also printed using a thermal inkjet printer on paper. Microfluidic channels were printed on paper to demonstrate the concept of paper-based bioassays as diagnostic devices. Paper is an ideal material for functional materials for functional bioactive surfaces.

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