The low permeability of many wood species causes significant problems during processing. Industrial methods used for increasing wood permeability reduce strength properties, are energy consuming, and are not viable economically. Destruction of pit membranes in wood cell walls can provide an increase in wood permeability without affecting wood strength properties. It can be accomplished using resonance applied to the pit membranes. Theoretical analysis and calculations have been performed to determine pit membrane (torus and margo) natural frequency. Membrane natural frequencies of bordered pits of Norway spruce are in the range of 3 to 11 MHz. Water in the pit chamber did not have a significant effect on the resonant frequency of the membrane. The main limitation of the amplitude of membrane fluctuations inside the pit chamber was the width of the chamber. Two methods to initiate resonance frequency for pit membrane destruction have been suggested, namely, alternating electric field application and microwave energy pulsation.